PXD006372

PXD006372 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 2
Description	Reactive oxygen species (ROS) are increasingly recognised as important regulators of cellular biology through the oxidative modification of protein cysteine residues. Comprehensive identification of redox-regulated proteins and cellular pathways are crucial to understand ROS-mediated events. Here, we present a new Stable Isotope Cysteine Labelling with IodoAcetamide (SICyLIA) MS-based proteomic workflow to assess protein cysteine oxidation in diverse cellular models and primary tissues. This approach informs on all possible cysteine oxidative modifications and achieves proteome-wide sensitivity with unprecedented depth without using enrichment steps. Our results suggest that acute and chronic oxidative stress cause metabolic adaptation through direct oxidation of metabolic and mitochondrial proteins. Analysis of chronically stressed fumarate hydratase-deficient mouse kidneys identified oxidation of proteins circulating in bio fluids, through which redox stress may affect whole-body physiology. Obtaining accurate peptide oxidation profiles from a complex organ using SICyLIA holds promise for future application to patient-derived samples in studies of human disease. PART 1 Hydrogen Peroxide PART 2 Primary immortalised kidney epithelial cells fumarate hydratase (FH) deficient PART 3 kidney tissues fumarate hydratase (FH) deficient
HostingRepository	PRIDE
AnnounceDate	2018-03-06
AnnouncementXML	Submission_2018-04-24_08:01:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Lilla
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-25 01:37:07	ID requested
1	2018-03-06 04:18:21	announced
⏵ 2	2018-04-24 08:01:05	announced	Updated publication reference for PubMed record(s): 29679077.

van der Reest J, Lilla S, Zheng L, Zanivan S, Gottlieb E, Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress. Nat Commun, 9(1):1581(2018) [pubmed]

curator keyword: Biological

submitter keyword: Oxidative stress, ROS, cysteine oxidation quantitation, Primary immortalised kidney epithelial cells fumarate hydratase (FH) deficient, Mouse kidney tissue fumarate hydratase (FH) deficient, High pH fractionation, Orbitrap HF, Proteome, Dimethyl labeling

Sara Rossana Zanivan
contact affiliation	CRUK - Beatson Institute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom
contact email	s.zanivan@beatson.gla.ac.uk
lab head
Sergio Lilla
contact affiliation	Beatson Institute for Cancer Research
contact email	s.Lilla@beatson.gla.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD006372

PRIDE project URI

• PRIDE
  1. PXD006372
     1. Label: PRIDE project
     2. Name: Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress, PART 2