PXD006371

PXD006371 is an original dataset announced via ProteomeXchange.

Title	Multiple Extracellular Vesicle Types in Peritoneal Dialysis Effluent are Prominent and Contain Known Biomarkers
Description	Proteomics-based analysis of Peritoneal Dialysis Effluent is a relatively new approach to studying the changes associated with PD. Recent studies have shown altered proteomic profiles in various conditions of PD patients, including uremia, diabetes, peritonitis, abnormal peritoneal transport function and with different peritoneal dialysis modality. However, these studies extracted proteins in PDE by precipitation techniques resulting in high abundant diffusible proteins from blood circulation which interfered with the identification of low abundant, locally secreted, yet important, biomarkers. we hypothesize that EVs are secreted from peritoneal resident cells and contain known biomarkers for PDE-related membrane injury. As a proof of concept, here we identified and characterized EVs from PDE of healthy PD patients using transmission electron microscopy (TEM), dynamic light scattering (DLS), and nanoparticle tracking analysis (NTA). Furthermore, we did proteomics and bioinformatics analyses to determine the number and type of proteins measurable within EVs isolated from the PDE using two methods of vesicle isolation.
HostingRepository	PRIDE
AnnounceDate	2018-08-14
AnnouncementXML	Submission_2019-02-12_08:19:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Poorichaya Somparn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-24 23:29:27	ID requested
1	2018-08-14 03:48:20	announced
⏵ 2	2019-02-12 08:19:54	announced	Updated publication reference for PubMed record(s): 28594924.

Pearson LJ, Klaharn IY, Thongsawang B, Manuprasert W, Saejew T, Somparn P, Chuengsaman P, Kanjanabuch T, Pisitkun T, Multiple extracellular vesicle types in peritoneal dialysis effluent are prominent and contain known biomarkers. PLoS One, 12(6):e0178601(2017) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biomedical

submitter keyword: Proteomics, extracellular vesicles, Peritoneal Dialysis

Trairak Pisitkun
contact affiliation	Systems Biology Center, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
contact email	Trairak.P@chula.ac.th
lab head
Poorichaya Somparn
contact affiliation	Chulalongkorn university
contact email	poorichaya.s@chula.ac.th
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD006371

PRIDE project URI

• PRIDE
  1. PXD006371
     1. Label: PRIDE project
     2. Name: Multiple Extracellular Vesicle Types in Peritoneal Dialysis Effluent are Prominent and Contain Known Biomarkers