PXD006365

PXD006365 is an original dataset announced via ProteomeXchange.

Title	Quantitative global and phosphoproteomic analysis to define the cellular role of AMPK β1-Ser108 phosphorylation
Description	AMP-activated protein kinase (AMPK) is a metabolic stress-sensing enzyme responsible for maintaining cellular energy homeostasis. Activation of AMPK by salicylate and the thienopyridone A-769662 is critically dependent on phosphorylation of Ser108 in the β1 regulatory subunit. We identified the autophagy initiator Unc-51-like kinase 1 (ULK1) as a β1-Ser108 kinase. ULK1 phosphorylation was specific to the β1-isoform and sensitised AMPK to salicylate and A-769662. To investigate the cellular fate of β1-Ser108 phosphorylation, we performed a stable isotope dimethyl labelling-based quantitative proteomic and phosphoproteomic analysis using lentivirus-transduced S108A and S108E mutants of β1 in β1/2-dKO iMEFs cells. Following 1 h phenformin (2 mM) treatment, β1-S108A and β1-S108E transduced iMEF lysates were compared to trace out the changes in proteome and phosphoprotome to decipher the possible cellular role of β1-Ser108 phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_14:00:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashfaqul Hoque
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-24 06:07:52	ID requested
⏵ 1	2018-10-24 14:00:30	announced

Dite TA, Ling NXY, Scott JW, Hoque A, Galic S, Parker BL, Ngoei KRW, Langendorf CG, O'Brien MT, Kundu M, Viollet B, Steinberg GR, Sakamoto K, Kemp BE, Oakhill JS, The autophagy initiator ULK1 sensitizes AMPK to allosteric drugs. Nat Commun, 8(1):571(2017) [pubmed]

curator keyword: Biological

submitter keyword: AMPK β1-Ser108, ULK1, autophagy

Jonathan S. Oakhill
contact affiliation	ARC Future Fellow Head, Metabolic Signalling Laboratory Protein Chemistry and Metabolism St. Vincent's Institute of Medical Research 9 Princes St, Fitzroy, Vic 3065, Australia
contact email	joakhill@svi.edu.au
lab head
Ashfaqul Hoque
contact affiliation	St Vincent's Institute of Medical Research (SVI)
contact email	ahoque@svi.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006365
     1. Label: PRIDE project
     2. Name: Quantitative global and phosphoproteomic analysis to define the cellular role of AMPK β1-Ser108 phosphorylation