<<< Full experiment listing

PXD006355

PXD006355 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein ID of ϕRP31
DescriptionJumbo phages ϕRP31/ϕRP12 infecting Ralstonia solanacearum were isolated in Thailand. Virion proteins of ϕRP31 separated by SDS-PAGE were visualized with Coomassie Brilliant Blue. The protein bands were excised from the gel, digested with trypsin, and analyzed by liquid chromatography-tandem mass spectrometry (LTQ Orbitrap XL). Assignment of tandem mass spectrometry data to tryptic peptides encoded by phage open reading frames was completed using an established procedure (Ahmad et al., 2014). Based on these genomic similarities, proteomic tree and gene phylogenies, we propose that ϕRP12 and ϕRP31 are new members of the group of ϕKZ-related phages.
HostingRepositoryjPOST
AnnounceDate2017-05-21
AnnouncementXMLSubmission_2022-09-18_02:19:00.482.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiyako Nakano
SpeciesList scientific name: phage;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-23 23:43:12ID requested
12017-05-21 01:38:33announced
22017-10-30 00:44:13announcedUpdated PubMed.
32018-09-19 00:12:31announcedUpdated CV, FTP location and Modification list.
42021-02-04 17:20:25announced2021-02-04: Updated FTP location.
52022-09-18 02:19:01announced2022-09-18: Updated FTP location.
Publication List
Matsui T, Yoshikawa G, Mihara T, Chatchawankanphanich O, Kawasaki T, Nakano M, Fujie M, Ogata H, Yamada T, Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase. Front Microbiol, 8():1010(2017) [pubmed]
Keyword List
submitter keyword: jumbo phage, ϕRP31, ϕKZ-like phages, Ralstonia solanacearum
Contact List
Miyako Nakano
lab head
Miyako Nakano
contact affiliationHiroshima University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000264/