PXD006354

PXD006354 is an original dataset announced via ProteomeXchange.

Title	Proteomic signature of environmental oxygen tension modulation of human macrophage polarization and functions
Description	Macrophages are innate immune cells characterized by their plasticity and their ability to react to various environmental stimuli. These cells are involved in a multiple number of tissular functions in homeostasis and pathological contexts. According to their environment these cells could be polarized toward different states of activation which determine their functional orientation. A large part of the macrophage biology field is devoted to better define what polarizations are, from a molecular point of view. It is now accepted that a multidimensional model of polarization is needed to grasp the broad phenotype repertoire depending on various environmental signals. Oxygen tension is one of these tissular environmental parameters. We designed this study to obtain a proteomic signature of various polarizations in human monocytes derived macrophages. We also seek to explore how environmental oxygen tension varying from an atmospheric composition (18.6% O2) to a “tissular normoxia” (3% O2) could modify our classification of macrophages’ polarization. We have obtained various polarization specific proteins and oxygen sensors for human macrophages. One example is arachidonate 15-lipoxygenase (ALOX15) which is a IL4/IL13 polarization specific proteins up regulated under low oxygen exposure associated to an increase of the phagocytosis rate of apoptotic cells. These results illustrate the necessity to take into account physicochemical parameters like oxygen when macrophage polarization is studied to correctly assess their functions in tissues.
HostingRepository	PRIDE
AnnounceDate	2017-09-11
AnnouncementXML	Submission_2017-09-11_04:57:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arnaud Millet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-21 12:26:09	ID requested
⏵ 1	2017-09-11 04:57:43	announced

Court M, Petre G, Atifi ME, Millet A, Proteomic Signature Reveals Modulation of Human Macrophage Polarization and Functions Under Differing Environmental Oxygen Conditions. Mol Cell Proteomics, 16(12):2153-2168(2017) [pubmed]

curator keyword: Biological

submitter keyword: Macrophages, Polarization, Oxygen, LC-MS/MS, Efferocytosis

Millet
contact affiliation	U1205 Inserm
contact email	arnaud.millet@inserm.fr
lab head
Arnaud Millet
contact affiliation	Inserm
contact email	arnaud.millet@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006354

PRIDE project URI

• PRIDE
  1. PXD006354
     1. Label: PRIDE project
     2. Name: Proteomic signature of environmental oxygen tension modulation of human macrophage polarization and functions