PXD006353

PXD006353 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An optimised method for CSF extracellular vesicle extraction and comparative proteomic analysis
Description	Extracellular vesicles (EVs) are implicated in the pathogenesis of neurodegenerative diseases and offer a source of biomarkers for diagnostic, stratification and therapeutic monitoring purposes. A major limitation in the development of specific biomarkers based on cerebrospinal fluid (CSF) EVs is the difficulty of extracting sufficient quantities of vesicles for discovery techniques such as proteomics. This study describes a novel application of a size-based extraction technique for CSF EVs using ultrafiltration liquid chromatography (UFLC), and comparison with the gold-standard ultracentrifugation (UC) method.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:38.875.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006353
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marie-Laëtitia Thézénas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-21 10:26:20	ID requested
1	2018-11-30 02:49:50	announced
⏵ 2	2024-10-22 04:36:39	announced	2024-10-22: Updated project metadata.

Publication List

10.1002/pmic.201800257;
10.6019/PXD006353;
Thompson AG, Gray E, Mager I, Fischer R, Th, é, z, é, nas ML, Charles PD, Talbot K, El Andaloussi S, Kessler BM, Wood M, Turner MR, UFLC-Derived CSF Extracellular Vesicle Origin and Proteome. Proteomics, 18(24):e1800257(2018) [pubmed]

10.1002/pmic.201800257;

10.6019/PXD006353;

Thompson AG, Gray E, Mager I, Fischer R, Th, é, z, é, nas ML, Charles PD, Talbot K, El Andaloussi S, Kessler BM, Wood M, Turner MR, UFLC-Derived CSF Extracellular Vesicle Origin and Proteome. Proteomics, 18(24):e1800257(2018) [pubmed]

Keyword List

curator keyword: Technical
submitter keyword: cerebrospinal fluid,Extracellular vesicles, LC-MS/MS

curator keyword: Technical

submitter keyword: cerebrospinal fluid,Extracellular vesicles, LC-MS/MS

Contact List

Pr. Benedikt Kessler
contact affiliation	University of Oxford, NDM Research Building, Target Discovery Institute, Kessler Group. OXFORD
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
Marie-Laëtitia Thézénas
contact affiliation	University of Oxford
contact email	marie-laetitia.thezenas@ndm.ox.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD006353
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD006353

PRIDE project URI

Repository Record List

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