PXD006352

PXD006352 is an original dataset announced via ProteomeXchange.

Title	Cross-species complementation reveals conserved functions for EARLY FLOWERING 3 between monocots and dicots.
Description	Plant responses to the environment are shaped by the external stimuli and internal signaling pathways. In both the model plant Arabidopsis thaliana and crop species, circadian clock factors have been identified as critical for growth, flowering and circadian rhythms. Outside of A. thaliana, however, little is known about the molecular function of clock genes. Therefore, we sought to compare the function of Brachypodium distachyon and Seteria viridis orthologs of EARLY FLOWERING3, a key clock gene in A. thaliana. To identify both cycling genes and putative ELF3 functional orthologs in S. viridis, a circadian RNA-seq dataset and online query tool (Diel Explorer) was generated as community resource to explore expression profiles of Setaria genes under constant conditions after photo- or thermo-entrainment. The function of ELF3 orthologs from A. thaliana, B. distachyon, and S. viridis were tested for complementation of an elf3 mutation in A. thaliana. Despite comparably low sequence identity versus AtELF3 (< 37%), both monocot orthologs were capable of rescuing hypocotyl elongation, flowering time and arrhythmic clock phenotypes. Molecular analysis using affinity purification and mass spectrometry to compare physical interactions also found that BdELF3 and SvELF3 could be integrated into similar complexes and networks as AtELF3, including forming a composite evening complex. Thus, we find that, despite 180 million years of separation, BdELF3 and SvELF3 can functionally complement loss of ELF3 at the molecular and physiological level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:09.961.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006352
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bradley Evans
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Phospho; Carboxymethyl; Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-21 08:07:58	ID requested
1	2018-01-08 01:26:58	announced
2	2018-05-23 01:56:18	announced	Updated publication reference for DOI(s): 10.1002/pld3.18.
⏵ 3	2023-11-14 08:49:10	announced	2023-11-14: Updated project metadata.

10.6019/PXD006352;

curator keyword: Biological

submitter keyword: Arabidopsis, AP-MS,Circadian Signaling, Orbitrap

Dmitri A. Nusinow
contact affiliation	Donald Danforth Plant Scinece Center
contact email	meter@danforthcenter.org
lab head
Bradley Evans
contact affiliation	Donald Danforth Plant Science Center
contact email	bevans@danforthcenter.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006352

PRIDE project URI

• PRIDE
  1. PXD006352
     1. Label: PRIDE project
     2. Name: Cross-species complementation reveals conserved functions for EARLY FLOWERING 3 between monocots and dicots.