PXD006348

PXD006348 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomic analysis of Brucella abortus and Brucella melitensis
Description	Brucella type strains (B. abortus 544 and B. melitensis 16 M), field-isolates (B. abortus T and B. melitensis C) were independently cultivated six times on Tryptic Soy Broth at 37 °C in the presence of 5% CO2 until mid-logarithmic phase. The cells were harvested by brief centrifugation and the bacteria was inactivated using recommended ethanol precipitation procedure. Following which the cells were lysed, proteins quantified and the differentially expressed proteins among B. abortus and B. melitensis were identified using liquid chromatography-mass spectrometry (LC/MS) based label free quantitative proteomics analysis.
HostingRepository	PRIDE
AnnounceDate	2020-06-11
AnnouncementXML	Submission_2020-06-11_15:30:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jayaseelan Murugaiyan
SpeciesList	scientific name: Brucella melitensis; NCBI TaxID: 29459; scientific name: Brucella abortus; NCBI TaxID: 235;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-21 00:21:25	ID requested
⏵ 1	2020-06-11 15:31:00	announced

Murugaiyan J, Eravci M, Weise C, Roesler U, Sprague LD, Neubauer H, Wareth G, . Biomolecules, 10(6):(2020) [pubmed]

ProteomeXchange project tag: ProteomeTools

curator keyword: Biological, Biomedical

submitter keyword: Brucella abortus, Brucella melitensis, comparative proteomic analysis, label free quantitative analysis, LTQ Orbitrap Velos mass spectrometer, Maxquant software, MaxQuant-Andromeda, Perseus software

Jayaseelan Murugaiyan
contact affiliation	Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany.
contact email	jayaseelan.murugaiyan@fu-berlin.de
lab head
Jayaseelan Murugaiyan
contact affiliation	Freie Universitaet-Berlin,Institute of Animal Hygiene and Environmental Health
contact email	jayaseelan.murugaiyan@fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD006348

PRIDE project URI

• PRIDE
  1. PXD006348
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomic analysis of Brucella abortus and Brucella melitensis