PXD006339

PXD006339 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS proteomics on glomeruli from individuals with extreme duration of type 1 diabetes
Description	Diabetic nephropathy (DN) is a major cause of end-stage renal disease and has limited therapeutic options to prevent its progression. To identify novel therapeutic strategies, protective factors for DN were studied using proteomics on glomeruli from 50-year medalists (individuals with extreme duration, ≥50 years, of diabetes in Joslin Medalist Study) without DN and those with histologic signs of DN. Many enzymes in the glycolytic, sorbitol, methylglyoxal and mitochondrial pathways were elevated in individuals without DN. We also identified that pyruvate kinase M2 (PKM2) expression and activity were upregulated. We then looked into the mechanisms and further validated the findings in vivo (Podocyte-specific Pkm2 KO mice model) and in vitro (cultured podocytes).
HostingRepository	PRIDE
AnnounceDate	2017-05-05
AnnouncementXML	Submission_2017-05-05_05:20:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	I-Hsien Wu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acrylamidyl cysteinyl; monohydroxylated residue; deamidated L-asparagine; deamidated L-glutamine
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-20 00:57:30	ID requested
⏵ 1	2017-05-05 05:20:42	announced

Qi W, Keenan HA, Li Q, Ishikado A, Kannt A, Sadowski T, Yorek MA, Wu IH, Lockhart S, Coppey LJ, Pfenninger A, Liew CW, Qiang G, Burkart AM, Hastings S, Pober D, Cahill C, Niewczas MA, Israelsen WJ, Tinsley L, Stillman IE, Amenta PS, Feener EP, Vander Heiden MG, Stanton RC, King GL, Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med, 23(6):753-762(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Type 1 Diabetes, T1DM, Diabetic Nephropathy, Glomeruli, 50-Year Medalist, Protective Factor, Kidney, LC-MS/MS

George King
contact affiliation	Joslin Diabetes Center, Harvard Medical School
contact email	george.king@joslin.harvard.edu
lab head
I-Hsien Wu
contact affiliation	Joslin Diabetes Center
contact email	i-hsien.wu@joslin.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006339

PRIDE project URI

• PRIDE
  1. PXD006339
     1. Label: PRIDE project
     2. Name: LC-MS/MS proteomics on glomeruli from individuals with extreme duration of type 1 diabetes