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PXD006330

PXD006330 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAdrenergic Receptor Stimulation Suppresses Metabolism in the Pancreatic β Cell
DescriptionGlucose-stimulated insulin secretion (GSIS) is suppressed through α-adrenergic receptor stimulation by catecholamines, epinephrine and norepinephrine, in pancreatic β-cells. Previous work has elucidated a bevy of adrenergic regulatory mechanisms beyond traditional Gi-coupled signaling including regulation of ion channels and interactions with exocytotic machinery. Glucose oxidation may also be an important site for adrenergic regulation of GSIS, but the link between epinephrine and glucose oxidation in β-cells is undefined. Here, we evaluate whether adrenergic stimulation decreases oxidative metabolism in β cells. Oxygen consumption rates were determined for Min6 and isolated rat islets in 20mM glucose complete media, then epinephrine was added at either 0 nM (vehicle control) or 100nM, followed by 10uM yohimbine (a selective Adrα2A antagonist). To identify glucose oxidation as the primary metabolic pathway affected by epinephrine, oxidation of 14C(U)-labeled glucose was determined in Min6 cells with epinephrine or vehicle. Oxygen consumption and glucose oxidation experiments were conducted in the presence of cAMP and insulin secretion blockers, respectively. Proteomics was performed on Min6 cells exposed to epinephrine for 4 hours and compared to controls. Epinephrine, but not vehicle, reduced (P<0.01) oxygen consumption rates in rat islets and Min6 cells to 64 ± 6% and 65 ± 1% of baseline, respectively, and yohimbine restored oxygen consumption to rates not different from baseline. In Min6 cells incubated with epinephrine rates of 14C glucose oxidation were reduced (P<0.01) 66 ± 4% compared to vehicle controls. These results demonstrate that acute epinephrine exposure suppresses glucose oxidation in β cells via the specific adrenergic receptor, Adrα2A, and indicate a new role for adrenergic regulation in GSIS.
HostingRepositoryPRIDE
AnnounceDate2019-11-08
AnnouncementXMLSubmission_2019-11-08_08:10:04.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKen Pendarvis
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentVelos Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-19 02:00:26ID requested
12019-11-08 08:10:05announced
Publication List
Kelly AC, Camacho LE, Pendarvis K, Davenport HM, Steffens NR, Smith KE, Weber CS, Lynch RM, Papas KK, Limesand SW, Adrenergic receptor stimulation suppresses oxidative metabolism in isolated rat islets and Min6 cells. Mol Cell Endocrinol, 473():136-145(2018) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: mouse, Min6, insulin, β-cells, pancreas, catecholamines, epinephrine, norepinephrine
Contact List
Sean Limesand
contact affiliationSchool of Comparative Animal and Biomedical Sciences University of Arizona Tucson, AZ USA
contact emaillimesand@ag.arizona.edu
lab head
Ken Pendarvis
contact affiliationVeterinary Science and Microbiology
contact emailjkpendarvis@email.arizona.edu
dataset submitter
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Dataset FTP location
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