PXD006328

PXD006328 is an original dataset announced via ProteomeXchange.

Title	Accumulation of small molecules and proteins in Arabidopsis thaliana root exudates by increased activity of stress-related mitogen-activated protein kinases.
Description	A delicate balance is required for plants to distinguish mutualist from parasitic organisms. Mitogen-activated protein kinase (MAPK) cascade is one of the signaling modules that mediate transduction of extracellular microbial signals into appropriate cellular response. Here we employ a transgenic system that simulates activation of two pathogen/stress-inducible MAPKs to study release of metabolites and proteins into root exudates. The premise is based on our previous proteomics study that suggests upregulation of secretory processes in this transgenic system. An advantage of this experimental set-up is the direct focus on MAPK-regulated processes without confounding complications of other signaling pathways activated by exposure to microbes or microbial molecules. Using non-targeted metabolomics and proteomics studies, we show that MAPK activation can indeed drive the exudation of dipeptides, defense-related metabolites and proteins in roots. However, the relative levels of other substances were decreased. This points to a bidirectional control of metabolite and protein release into the apoplast. The putative roles for some of the identified exudated metabolites and proteins are discussed with respect to possible antimicrobial/defense or allelopathic properties. Overall, our findings demonstrate that MAPKs regulate the composition of apoplastic root metabolites and proteins, presumably to influence the plant-microbe interactions in the rhizosphere.
HostingRepository	PRIDE
AnnounceDate	2017-07-14
AnnouncementXML	Submission_2017-09-12_02:53:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006328
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wolfgang Hoehenwarter
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-19 01:42:05	ID requested
1	2017-07-14 06:16:00	announced
⏵ 2	2017-09-12 02:53:53	announced	Updated publication reference for PubMed record(s): 28785276.

Strehmel N, Hoehenwarter W, M, ö, nchgesang S, Majovsky P, Kr, ü, ger S, Scheel D, Lee J, Root Exudates. Front Plant Sci, 8():1292(2017) [pubmed]

curator keyword: Biological

submitter keyword: root exudates, roots, metabolite profiling, plant defense, signaling, Arabidopsis thaliana, metabolomics, proteomics

Wolfgang Hoehenwarter
contact affiliation	Proteome Analytics Leibniz Institute of Plant Biochemistry Weinberg 3 Halle 06124, Germany
contact email	wolfgang.hoehenwarter@ipb-halle.de
lab head
Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006328
     1. Label: PRIDE project
     2. Name: Accumulation of small molecules and proteins in Arabidopsis thaliana root exudates by increased activity of stress-related mitogen-activated protein kinases.