PXD006326 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling |
Description | GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs) play essential roles in regulating the activity of small GTPases. Several GAPs and GEFs have been shown to be present at the postsynaptic density (PSD) within excitatory glutamatergic neurons and regulate the activity of glutamate receptors. However, it is not known how synaptic GAP and GEF proteins are organized within the PSD signaling machinery, if they have overlapping interaction networks, or if they associate with proteins implicated in contributing to psychiatric disease. Here, we determine the interactomes of three interacting GAP/GEF proteins at the PSD, including the RasGAP Syngap1, the ArfGAP Agap2, and the RhoGEF Kalirin. We describe the functional properties of each interactome and show that these GAP/GEF proteins are highly associated with and cluster other proteins directly involved in GTPase signaling mechanisms. We also utilize Agap2 as an example of GAP/GEFs localized within multiple neuronal compartments and determine additional interactions involving Agap2 outside of the PSD. Functional analysis of PSD and non-PSD interactomes illustrates both common and unique functions of Agap2 determined by its subcellular location. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:33:21.653.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Brent Wilkinson |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ FT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-19 01:10:12 | ID requested | |
1 | 2022-02-22 03:50:47 | announced | |
⏵ 2 | 2024-10-22 05:33:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
Wilkinson B, Li J, Coba MP, Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep, 7(1):5272(2017) [pubmed] |
10.1038/s41598-017-05588-3; |
Keyword List
curator keyword: Biological |
submitter keyword: Mouse, Neuron, GTP |
Contact List
Marcelo Coba |
contact affiliation | University of Southern California |
contact email | coba@usc.edu |
lab head | |
Brent Wilkinson |
contact affiliation | University of Southern California |
contact email | brentwil@usc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006326
- Label: PRIDE project
- Name: Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling