PXD006326

PXD006326 is an original dataset announced via ProteomeXchange.

Title	Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling
Description	GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs) play essential roles in regulating the activity of small GTPases. Several GAPs and GEFs have been shown to be present at the postsynaptic density (PSD) within excitatory glutamatergic neurons and regulate the activity of glutamate receptors. However, it is not known how synaptic GAP and GEF proteins are organized within the PSD signaling machinery, if they have overlapping interaction networks, or if they associate with proteins implicated in contributing to psychiatric disease. Here, we determine the interactomes of three interacting GAP/GEF proteins at the PSD, including the RasGAP Syngap1, the ArfGAP Agap2, and the RhoGEF Kalirin. We describe the functional properties of each interactome and show that these GAP/GEF proteins are highly associated with and cluster other proteins directly involved in GTPase signaling mechanisms. We also utilize Agap2 as an example of GAP/GEFs localized within multiple neuronal compartments and determine additional interactions involving Agap2 outside of the PSD. Functional analysis of PSD and non-PSD interactomes illustrates both common and unique functions of Agap2 determined by its subcellular location.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:21.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brent Wilkinson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-19 01:10:12	ID requested
1	2022-02-22 03:50:47	announced
⏵ 2	2024-10-22 05:33:22	announced	2024-10-22: Updated project metadata.

Wilkinson B, Li J, Coba MP, Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep, 7(1):5272(2017) [pubmed]

10.1038/s41598-017-05588-3;

curator keyword: Biological

submitter keyword: Mouse, Neuron, GTP

Marcelo Coba
contact affiliation	University of Southern California
contact email	coba@usc.edu
lab head
Brent Wilkinson
contact affiliation	University of Southern California
contact email	brentwil@usc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006326

PRIDE project URI

• PRIDE
  1. PXD006326
     1. Label: PRIDE project
     2. Name: Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling