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PXD006326

PXD006326 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSynaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling
DescriptionGTPase-activating proteins (GAPs) and guanine exchange factors (GEFs) play essential roles in regulating the activity of small GTPases. Several GAPs and GEFs have been shown to be present at the postsynaptic density (PSD) within excitatory glutamatergic neurons and regulate the activity of glutamate receptors. However, it is not known how synaptic GAP and GEF proteins are organized within the PSD signaling machinery, if they have overlapping interaction networks, or if they associate with proteins implicated in contributing to psychiatric disease. Here, we determine the interactomes of three interacting GAP/GEF proteins at the PSD, including the RasGAP Syngap1, the ArfGAP Agap2, and the RhoGEF Kalirin. We describe the functional properties of each interactome and show that these GAP/GEF proteins are highly associated with and cluster other proteins directly involved in GTPase signaling mechanisms. We also utilize Agap2 as an example of GAP/GEFs localized within multiple neuronal compartments and determine additional interactions involving Agap2 outside of the PSD. Functional analysis of PSD and non-PSD interactomes illustrates both common and unique functions of Agap2 determined by its subcellular location.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:33:21.653.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrent Wilkinson
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ FT
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-19 01:10:12ID requested
12022-02-22 03:50:47announced
22024-10-22 05:33:22announced2024-10-22: Updated project metadata.
Publication List
Wilkinson B, Li J, Coba MP, Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep, 7(1):5272(2017) [pubmed]
10.1038/s41598-017-05588-3;
Keyword List
curator keyword: Biological
submitter keyword: Mouse, Neuron, GTP
Contact List
Marcelo Coba
contact affiliationUniversity of Southern California
contact emailcoba@usc.edu
lab head
Brent Wilkinson
contact affiliationUniversity of Southern California
contact emailbrentwil@usc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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