Updated publication reference for PubMed record(s): 29176891. Leishmaniasis is an antropozoonosis caused by Leishmania parasites that affects around 12 million people in 98 different countries. The disease has different clinical forms, which depend mainly on the parasite genetics and on the immunologic status of the host. The promastigote form of the parasite is transmitted by an infected female phlebotomine sand fly, is internalized by phagocytic cells, mainly macrophages, and converts into amastigotes which replicate inside these cells. Macrophages are important cells of the immune system, capable of efficiently killing intracellular pathogens. However, Leishmania, can evade these mechanisms due to expression of virulence factors. Different strains of the same Leishmania species may have different infectivity and metastatic phenotypes in vivo. In the present work, we show that parasites from LV79 and PH8 strains have different lesion development in BALB/c and C57BL/6 mouse strains. The comparison of the proteomes of lesion-derived amastigotes from the two strains identified proteins such as CPx, SOD, HSP70 and GP63 as differentially expressed. The expression profile of all proteins and of the differentially expressed ones precisely classified PH8 and LV79 samples, indicating that the two strains are highly divergent and that protein expression correlate with their phenotypes.