PXD006321

PXD006321 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of amastigotes from Leishmania (L.) amazonensis LV79 and PH8 strains reveals molecular traits associated with the virulence phenotype
Description	Leishmaniasis is an antropozoonosis caused by Leishmania parasites that affects around 12 million people in 98 different countries. The disease has different clinical forms, which depend mainly on the parasite genetics and on the immunologic status of the host. The promastigote form of the parasite is transmitted by an infected female phlebotomine sand fly, is internalized by phagocytic cells, mainly macrophages, and converts into amastigotes which replicate inside these cells. Macrophages are important cells of the immune system, capable of efficiently killing intracellular pathogens. However, Leishmania, can evade these mechanisms due to expression of virulence factors. Different strains of the same Leishmania species may have different infectivity and metastatic phenotypes in vivo. In the present work, we show that parasites from LV79 and PH8 strains have different lesion development in BALB/c and C57BL/6 mouse strains. The comparison of the proteomes of lesion-derived amastigotes from the two strains identified proteins such as CPx, SOD, HSP70 and GP63 as differentially expressed. The expression profile of all proteins and of the differentially expressed ones precisely classified PH8 and LV79 samples, indicating that the two strains are highly divergent and that protein expression correlate with their phenotypes.
HostingRepository	PRIDE
AnnounceDate	2017-11-16
AnnouncementXML	Submission_2017-12-11_03:55:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebeca Sakuma
SpeciesList	scientific name: Leishmania ; NCBI TaxID: 38568;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-18 07:39:25	ID requested
1	2017-11-15 23:44:35	announced
⏵ 2	2017-12-11 03:55:49	announced	Updated publication reference for PubMed record(s): 29176891.

de Rezende E, Kawahara R, Pe, ñ, a MS, Palmisano G, Stolf BS, Quantitative proteomic analysis of amastigotes from Leishmania (L.) amazonensis LV79 and PH8 strains reveals molecular traits associated with the virulence phenotype. PLoS Negl Trop Dis, 11(11):e0006090(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Leishmania amazonensis, virulence, proteome comparison, clustering, lesion-derived amastigotes

Beatriz S. Stolf
contact affiliation	Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
contact email	bstolf@usp.br
lab head
Rebeca Sakuma
contact affiliation	University of Sao Paulo
contact email	rebecasakuma@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006321

PRIDE project URI

• PRIDE
  1. PXD006321
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of amastigotes from Leishmania (L.) amazonensis LV79 and PH8 strains reveals molecular traits associated with the virulence phenotype