PXD006317

PXD006317 is an original dataset announced via ProteomeXchange.

Title	A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B-cells
Description	Epstein Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B-cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain novel insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B-cell proteome and EBV virome. Our approach revealed EBV-induced remodelling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B-cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi sarcoma associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.
HostingRepository	PRIDE
AnnounceDate	2017-05-19
AnnouncementXML	Submission_2017-05-22_00:10:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Weekes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-18 05:25:35	ID requested
1	2017-05-19 01:20:52	announced
⏵ 2	2017-05-22 00:10:26	announced	Updated publication reference for PubMed record(s): 28514666.

Ersing I, Nobre L, Wang LW, Soday L, Ma Y, Paulo JA, Narita Y, Ashbaugh CW, Jiang C, Grayson NE, Kieff E, Gygi SP, Weekes MP, Gewurz BE, A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells. Cell Rep, 19(7):1479-1493(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Epstein-Barr virus, herpesvirus, lytic replication, quantitative proteomics, tandem mass tag, host-pathogen interaction, immune evasion, B-cell receptor, complement, viral evasion

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY
contact email	mpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006317
     1. Label: PRIDE project
     2. Name: A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B-cells