PXD006308

PXD006308 is an original dataset announced via ProteomeXchange.

Title	COMPARISON OF RABBIT SEMINAL PLASMA PROTEOME BETWEEN GENETIC LINES AND SEASONS
Description	Seminal plasma contributes to the safe environment for sperm maturation, sperm viability and fertilization in mammals (Kumar 2013). Moreover, seminal plasma is a promising source for the study of potential reproductive biomarkers, because it is a complex mixture of secretions from testis, epididymis and male accessory sex glands (González-Cadavid et al. 2014). The protein composition of mammalian seminal plasma varies among species, and has important effects on sperm function (Mortarino et al. 1998). In rabbits, seminal plasma has a positive effect in maintaining sperm motility and viability during in vitro storage (Castellini et al. 2000). To study proteins on a large scale, the application of a proteomic approach is mandatory (Dietrich 2014). Rabbit seminal plasma proteome has been proved different between rabbit genetic line A (maternal) and R (growth) (Viudes-de-Castro et al. (2004), Safaa et al. (2008), Casares-Crespo 2016) and between seasons (Casares-Crespo 2016). In these previous studies a traditional 1 dimension polyacrylamide gel was done, identifying only major proteins visible after Coomassie Colloidal Blue staining and obtaining the relative quantity of these protein bands. On the contrary, new approaches such as liquid chromatography in-gel or in-solution digestion coupled with mass spectrometric analysis are a powerful, yet relatively uncomplicated strategy to identify and characterize proteins. Mass spectrometry-based proteomics has become the tool of choice for identifying and quantifying the proteome of an organism (Karpievitch 2010). Therefore, the present study was conducted to describe the seminal plasma proteome of rabbits, compare the differentially expressed seminal plasma proteins between genetic lines A and R and study the effect of season in rabbit seminal plasma proteome.
HostingRepository	PRIDE
AnnounceDate	2018-01-04
AnnouncementXML	Submission_2018-01-04_02:44:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006308
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-17 01:49:01	ID requested
⏵ 1	2018-01-04 02:44:55	announced

Casares-Crespo L, Fern, á, ndez-Serrano P, Vicente JS, Marco-Jim, é, nez F, Viudes-de-Castro MP, Rabbit seminal plasma proteome: The importance of the genetic origin. Anim Reprod Sci, 189():30-42(2018) [pubmed]

curator keyword: Biological

submitter keyword: rabbit, seminal plasma, proteome, genetic line, season, LC-MS/MS

María Pilar Viudes-de-Castro
contact affiliation	Centro de Investigación y Tecnología Animal-Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain.
contact email	viudes_mar@gva.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006308
     1. Label: PRIDE project
     2. Name: COMPARISON OF RABBIT SEMINAL PLASMA PROTEOME BETWEEN GENETIC LINES AND SEASONS