PXD006301

PXD006301 is an original dataset announced via ProteomeXchange.

Title	Expression of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta
Description	Plants and algae have developed various light harvesting mechanisms for optimal delivery of excitation energy to the photosystems. The cryptophyte algae have evolved a novel soluble light-harvesting antenna utilizing phycobilin pigments to complement the membrane-intrinsic Chl a/c-binding LHC antenna. This new antenna consists of the plastid-encoded beta-subunit, a relict of the ancestral phycobilisome, and a novel nuclear-encoded -subunit unique to cryptophytes. Together, these proteins form the active tetramer, which consists of one alpha 1 and one alpha 2 and two beta susbunits. In all cryptophyte algae investigated so far, the alpha-subunits have duplicated and diversified into a large gene family. Although there is transcriptional evidence for expression of all these genes, the x-ray structures determined to date suggest that only two of the -subunit genes might be significantly expressed at protein level. Using proteomics, we show that phycoerythrin 545 (PE545) of Guillardia theta, the only cryptophyte with a sequenced genome, all 20 alpha-subunits are expressed when the algae grow under white light. Their relative expression levels depend on the intensity of the growth light, but there is no evidence for a specific light-dependent regulation of individual members of the alpha-subunit family under the growth conditions applied. Subunit GtcpeA10 seems to be a special member of the alpha-subunit family, because it consists of two similar N- and C-terminal domains, which likely are the result of a partial gene duplication.
HostingRepository	PRIDE
AnnounceDate	2017-06-20
AnnouncementXML	Submission_2017-06-20_04:27:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006301
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Kieselbach
SpeciesList	scientific name: Guillardia theta CCMP2712; NCBI TaxID: 905079;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-13 08:28:30	ID requested
⏵ 1	2017-06-20 04:27:30	announced

Kieselbach T, Cheregi O, Green BR, Funk C, Proteomic analysis of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta cultured under different light intensities. Photosynth Res, 135(1-3):149-163(2018) [pubmed]

curator keyword: Biological

submitter keyword: Plant, cryptophyte, Gulillarida theta, phycobiliprotein, LC_MS/MS, proteomics

Thomas Kieselbach
contact affiliation	Umea University, Department of Chemistry, 90187 Umea, Sweden
contact email	thomas.kieselbach@chem.umu.se
lab head
Thomas Kieselbach
contact affiliation	Biochemistry
contact email	thomas.kieselbach@chem.umu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006301
     1. Label: PRIDE project
     2. Name: Expression of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta