PXD006301 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Expression of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta |
Description | Plants and algae have developed various light harvesting mechanisms for optimal delivery of excitation energy to the photosystems. The cryptophyte algae have evolved a novel soluble light-harvesting antenna utilizing phycobilin pigments to complement the membrane-intrinsic Chl a/c-binding LHC antenna. This new antenna consists of the plastid-encoded beta-subunit, a relict of the ancestral phycobilisome, and a novel nuclear-encoded -subunit unique to cryptophytes. Together, these proteins form the active tetramer, which consists of one alpha 1 and one alpha 2 and two beta susbunits. In all cryptophyte algae investigated so far, the alpha-subunits have duplicated and diversified into a large gene family. Although there is transcriptional evidence for expression of all these genes, the x-ray structures determined to date suggest that only two of the -subunit genes might be significantly expressed at protein level. Using proteomics, we show that phycoerythrin 545 (PE545) of Guillardia theta, the only cryptophyte with a sequenced genome, all 20 alpha-subunits are expressed when the algae grow under white light. Their relative expression levels depend on the intensity of the growth light, but there is no evidence for a specific light-dependent regulation of individual members of the alpha-subunit family under the growth conditions applied. Subunit GtcpeA10 seems to be a special member of the alpha-subunit family, because it consists of two similar N- and C-terminal domains, which likely are the result of a partial gene duplication. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:36:42.636.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD006301 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Thomas Kieselbach |
SpeciesList | scientific name: Guillardia theta CCMP2712; NCBI TaxID: 905079; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-13 08:28:30 | ID requested | |
1 | 2017-06-20 04:27:30 | announced | |
⏵ 2 | 2024-10-22 04:36:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.6019/PXD006301; |
Kieselbach T, Cheregi O, Green BR, Funk C, Proteomic analysis of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta cultured under different light intensities. Photosynth Res, 135(1-3):149-163(2018) [pubmed] |
10.1007/s11120-017-0400-0; |
Keyword List
curator keyword: Biological |
submitter keyword: Plant, proteomics, phycobiliprotein, Gulillarida theta, cryptophyte, LC_MS/MS |
Contact List
Thomas Kieselbach |
contact affiliation | Umea University, Department of Chemistry, 90187 Umea, Sweden |
contact email | thomas.kieselbach@chem.umu.se |
lab head | |
Thomas Kieselbach |
contact affiliation | Umea University, Dept. of Chemistry |
contact email | thomas.kieselbach@umu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006301
- Label: PRIDE project
- Name: Expression of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta