PXD006295

PXD006295 is an original dataset announced via ProteomeXchange.

Title	SWATH-MS dataset of Garcinia mangostana pericarp’s proteome across ripening stages
Description	Data provided is derived from our research on comparing protein expression profiling of Garcinia mangostana (G. mangostana) Linn. on different ripening stages. Proteins were extracted from the pericarp of G. mangostana at three points of ripening stages (stage 0, 2 and 6) and digested with trypsin before being analyzed using 1 Dimensional and 2 Dimensional Information Dependent Acquisition Liquid Chromatography (1D and 2D IDA LC) coupled with sequential window acquisition of all theoretical fragment ion spectra (SWATH) analysis. This approach enable us to identify and relatively quantify protein in complex mixture. Protein identification was performed by matching tandem mass (MS/MS) spectra from 1D and 2D IDA to in-house-generated and UniProt databases using a database search algorithm software ProteinPilot (v4.2) (AB Sciex). Protein quantification was performed by analyzing protein peak areas extracted from SWATH analysis data sets using PeakView (v2.1) (AB Sciex) software. Finally, protein peaks were subjected to statistical analysis to compare relative protein peak areas between the sample groups.
HostingRepository	PRIDE
AnnounceDate	2021-05-24
AnnouncementXML	Submission_2021-05-24_04:15:24.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wan Aizat
SpeciesList	scientific name: Garcinia mangostana; NCBI TaxID: 58228;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-13 07:42:45	ID requested
⏵ 1	2021-05-24 04:15:24	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Garcinia mangostana Linn., Protein profile, Mass spectrometry data, Quantitative proteomics, SWATH analysis

Dr. Wan Mohd Aizat
contact affiliation	Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia
contact email	wma@ukm.edu.my
lab head
Wan Aizat
contact affiliation	Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia
contact email	wma@ukm.edu.my
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD006295

PRIDE project URI

• PRIDE
  1. PXD006295
     1. Label: PRIDE project
     2. Name: SWATH-MS dataset of Garcinia mangostana pericarp’s proteome across ripening stages