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PXD006294 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNew insight into interactome of plant NDPKs
DescriptionInteractions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity-purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, to analyse protein‒metabolite and protein‒protein interactions simultaneously in plant systems. More specifically, we examined protein and small-molecule partners of the three (of five) nucleoside diphosphate kinases present in the Arabidopsis genome (NDPK1‒3). The bona fide role of NDPKs is the exchange of terminal phosphate groups between nucleoside di- (NDP) and triphosphates (NTP). However, other functions have been reported, which likely depend on both the proteins and small molecules specifically interacting with the NDPK. Using our approach we identified 23, 17, and 8 novel protein partners of NDPK1, NDPK2, and NDPK3, respectively, with nucleotide-dependent proteins such as actin and adenosine kinase 2 being enriched. Particularly interesting, however, was the co-elution of glutathione S-transferases (GSTs) and reduced glutathione (GSH) with the affinity-purified NDPK1 complexes. Following up on this finding, we could demonstrate that NDPK1 undergoes glutathionylation, opening a new paradigm of NDPK regulation in plants. The described results extend our knowledge of NDPKs, the key enzymes regulating NDP/NTP homeostasis.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarcin Luzarowski
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-13 07:34:21ID requested
12019-10-09 00:36:07announced
Publication List
Luzarowski M, Kosmacz M, Sokolowska E, Jasinska W, Willmitzer L, Veyel D, Skirycz A, Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases. J Exp Bot, 68(13):3487-3499(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: A. thaliana, affinity purification, glutathionylation, metabolites, NDPK, plant, protein‒metabolite interactions, protein‒protein interactions
Contact List
Lothar Willmitzer
contact affiliationAG Willmitzer, Max Planck Institute of Molecular Plants Physiology, Potsdam-Golm, Germany
contact emailWillmitzer@mpimp-golm.mpg.de
lab head
Marcin Luzarowski
contact affiliationMax Planck Institute of Molecular Plant Physiology
contact emailluzarowski@mpimp-golm.mpg.de
dataset submitter
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