PXD006294

PXD006294 is an original dataset announced via ProteomeXchange.

Title	New insight into interactome of plant NDPKs
Description	Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity-purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, to analyse protein‒metabolite and protein‒protein interactions simultaneously in plant systems. More specifically, we examined protein and small-molecule partners of the three (of five) nucleoside diphosphate kinases present in the Arabidopsis genome (NDPK1‒3). The bona fide role of NDPKs is the exchange of terminal phosphate groups between nucleoside di- (NDP) and triphosphates (NTP). However, other functions have been reported, which likely depend on both the proteins and small molecules specifically interacting with the NDPK. Using our approach we identified 23, 17, and 8 novel protein partners of NDPK1, NDPK2, and NDPK3, respectively, with nucleotide-dependent proteins such as actin and adenosine kinase 2 being enriched. Particularly interesting, however, was the co-elution of glutathione S-transferases (GSTs) and reduced glutathione (GSH) with the affinity-purified NDPK1 complexes. Following up on this finding, we could demonstrate that NDPK1 undergoes glutathionylation, opening a new paradigm of NDPK regulation in plants. The described results extend our knowledge of NDPKs, the key enzymes regulating NDP/NTP homeostasis.
HostingRepository	PRIDE
AnnounceDate	2019-10-09
AnnouncementXML	Submission_2019-10-09_00:36:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcin Luzarowski
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-13 07:34:21	ID requested
⏵ 1	2019-10-09 00:36:07	announced

Luzarowski M, Kosmacz M, Sokolowska E, Jasinska W, Willmitzer L, Veyel D, Skirycz A, Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases. J Exp Bot, 68(13):3487-3499(2017) [pubmed]

curator keyword: Biological

submitter keyword: A. thaliana, affinity purification, glutathionylation, metabolites, NDPK, plant, protein‒metabolite interactions, protein‒protein interactions

Lothar Willmitzer
contact affiliation	AG Willmitzer, Max Planck Institute of Molecular Plants Physiology, Potsdam-Golm, Germany
contact email	Willmitzer@mpimp-golm.mpg.de
lab head
Marcin Luzarowski
contact affiliation	Max Planck Institute of Molecular Plant Physiology
contact email	luzarowski@mpimp-golm.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006294
     1. Label: PRIDE project
     2. Name: New insight into interactome of plant NDPKs