PXD006285
PXD006285 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomic analysis of Staphylococcus aureus |
| Description | S. aureus is one of the major human pathogens that greatly impacts individuals and causes a variety of illnesses ranging from minor skin infections to life-threatening diseases, such as endocarditis, pneumonia, septicemia and toxic shock syndrome. Stk1/Stp1 are particular important for S. aureus pathogenesis and survival as they are thought to participate in regulating virulence, cell wall structure and antibiotic resistance. Understanding how Ser/Thr phosphorylation regulates virulence and antibiotic resistance will provide foundation for the development of novel therapeutic strategies against S. aureus infection. The phosphoproteomic analysis of Staphylococcus aureus Newman strain led to the identification of 76 peptides with mapped phosphorylation sites belonging to 29 distinct proteins. In the case of Stk1, only one peptide (residues 157-182), with the sequence ALSETSLTQTNHVLGTVQYFSPEQAK, was predicted to have a cluster of six phosphorylation sites (Ser159, Thr161, Ser162, Thr164, Thr166 and Thr172). This segment overlaps with the sequence of kinase activation loop (residues 154-176) in Stk1. Further biochemical studies revealed cis autophosphorylation of Thr172 in the GT/S motif is necessary for self-activation and kinase activity of Stk1, whereas the trans autophosphorylation of other activation loop serines/threonins are essential for the optimal kinase activity of Stk1. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:01:46.226.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Weihao Zheng |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | phosphorylated residue |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-04-11 00:05:22 | ID requested | |
| 1 | 2018-10-24 14:19:53 | announced | |
| ⏵ 2 | 2024-10-22 04:01:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.3389/fmicb.2018.00758; |
| Zheng W, Cai X, Li S, Li Z, . Front Microbiol, 9():758(2018) [pubmed] |
Keyword List
| curator keyword: Biological, Biomedical |
| submitter keyword: phosphoproteomic, Stp1, Stk1,Staphylococcus aureus |
Contact List
| Zigang Li | |
|---|---|
| contact affiliation | School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen, China 518055 |
| contact email | lizg@pkusz.edu.cn |
| lab head | |
| Weihao Zheng | |
| contact affiliation | Peking University Shenzhen Graduate School |
| contact email | whzheng@pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006285 |
| PRIDE project URI |
Repository Record List
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