PXD006280 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The nuclear receptor ERbeta engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading |
Description | Background: The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNAsilencing pathways, exerting a pivotal role in microRNA maturation and activity, that in the cell nucleus is able to modulate chromatin remodeling, transcriptional gene regulation and RNA splicing. The Estrogen Receptor beta (ERβ), a member of the nuclear receptor superfamily of trancriptional regulators, is endowed with oncosuppressive activities, antagonizing hormone-induced carcinogenesis and inhibiting growth and oncogenic functions in luminal-like breast cancers (BCs), where its expression correlates with a better prognosis of the disease. Results: Applying interaction proteomics coupled to mass spectrometry (MS) to characterize nuclear factors cooperating with ERβ in gene regulation, we identified AGO2 as a novel partner of ERβ in human BC cells. ERβ-AGO2 association was confirmed in vitro and in vivo both in the nucleus and cytoplasm. ChIP-Seq demonstrated AGO2 association to a large number of ERβ binding sites, and total and nascent RNA-Seq in ERβ+ vs ERβ- cells, and before and after AGO2 knock-down in ERβ+ cells, revealed a widespread involvement of this factor in ERβ-mediated regulation of gene transcription rate and RNA splicing. Moreover, isolation and sequencing by RIP-Seq of ERβ-associated long and small RNAs in the cytoplasm suggested involvement of the nuclear receptor in RISC loading, indicating that it may able to control directly also mRNA translation efficiency and stability. Conclusions: These results demonstrate that AGO2 is a pleiotropic functional partner of ERβ in BC cells, indicating that both factors are endowed with multiple roles in the control of BC cell functions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:01:51.181.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tuula Nyman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-10 05:04:12 | ID requested | |
1 | 2017-09-20 00:58:43 | announced | |
2 | 2017-10-16 06:17:01 | announced | Updated publication reference for PubMed record(s): 29017520. |
3 | 2018-03-12 02:29:18 | announced | Updated publication reference for PubMed record(s): 29017520. |
⏵ 4 | 2024-10-22 04:02:01 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tarallo R, Giurato G, Bruno G, Ravo M, Rizzo F, Salvati A, Ricciardi L, Marchese G, Cordella A, Rocco T, Gigantino V, Pierri B, Cimmino G, Milanesi L, Ambrosino C, Nyman TA, Nassa G, Weisz A, engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol, 18(1):189(2017) [pubmed] |
10.1038/sdata.2018.31; |
Giurato G, Nassa G, Salvati A, Alexandrova E, Rizzo F, Nyman TA, Weisz A, Tarallo R, interactome in human breast cancer cells. Sci Data, 5():180031(2018) [pubmed] |
10.1186/s13059-017-1321-0; |
Keyword List
curator keyword: Biomedical |
submitter keyword: AP-MS,ERbeta receptor, AGO2 |
Contact List
Tuula Nyman |
contact affiliation | Head of Proteomics, Group leader |
contact email | tuula.nyman@medisin.uio.no |
lab head | |
Tuula Nyman |
contact affiliation | University of Oslo |
contact email | tuula.nyman@medisin.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006280
- Label: PRIDE project
- Name: The nuclear receptor ERbeta engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading