PXD006277

PXD006277 is an original dataset announced via ProteomeXchange.

Title	Developmental profile of core postsynaptic density protein complexes integrates signaling components of brain disorders
Description	The postsynaptic density (PSD) contains a collection of scaffold proteins used for the assembly of synaptic signaling complexes and disruption of this signaling machinery might be implicated in a variety of brain disorders. However, it is not known how the core-scaffold machinery associates this collection of proteins through development and how proteins coding for genes involved in psychiatric and other brain disorders are distributed through spatio-temporal protein complexes. Here, using immunopurification, proteomics, bioinformatics and mouse genetics, we isolated 2876 proteins across 41 in-vivo protein complexes and determined their protein domain composition, correlation to gene expression levels, and developmental integration to the PSD. We defined major protein clusters for enrichment of schizophrenia (SCZ), autism spectrum disorders (ASD), developmental delay (DD), and intellectual disability (ID) risk factors at embryonic day 14 and the adult PSD. These protein complexes contained a discrete number of protein domains defining molecular functions. Mutations in highly-connected nodes alter protein-protein interactions that modulate the assembly of macromolecular complexes enriched in disease risk candidates. These results were integrated into a software platform: Synaptic Protein/Pathways Resource (SyPPRes), enabling the prioritization of brain disease risk factors and their placement within synaptic protein interaction networks.
HostingRepository	PRIDE
AnnounceDate	2017-08-22
AnnouncementXML	Submission_2017-08-22_05:14:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brent Wilkinson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-10 03:33:53	ID requested
⏵ 1	2017-08-22 05:14:51	announced

Li J, Zhang W, Yang H, Howrigan DP, Wilkinson B, Souaiaia T, Evgrafov OV, Genovese G, Clementel VA, Tudor JC, Abel T, Knowles JA, Neale BM, Wang K, Sun F, Coba MP, Spatiotemporal profile of postsynaptic interactomes integrates components of complex brain disorders. Nat Neurosci, 20(8):1150-1161(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: synaptic, PSD, neuron

Marcelo Coba
contact affiliation	University of Southern California
contact email	coba@usc.edu
lab head
Brent Wilkinson
contact affiliation	University of Southern California
contact email	brentwil@usc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006277

PRIDE project URI

• PRIDE
  1. PXD006277
     1. Label: PRIDE project
     2. Name: Developmental profile of core postsynaptic density protein complexes integrates signaling components of brain disorders