PXD006274

PXD006274 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Colwellia psychrerythraea grown at multiple subzero temperatures
Description	The mechanisms that allow psychrophilic bacteria to remain metabolically active at subzero temperatures result from form and function of their proteins. We present first proteomic evidence of physiological changes of the marine psychrophile Colwellia psychrerythraea 34H (Cp34H) after exposure to subzero temperatures (-1, and -10°C in ice) through 8 weeks. Protein abundance was compared between different treatments to understand the effects of temperature and time, independently and jointly, within cells transitioning to, and being maintained in ice. Parallel [3H]-leucine and [3H]-thymidine incubations indicated active protein and DNA synthesis to -10°C. Mass spectrometry-based proteomics identified 1763 proteins across four experimental treatments. Proteins involved in osmolyte regulation and polymer secretion were found constitutively present across all treatments, suggesting that they are required for metabolic success below 0°C. Differentially abundant protein groups indicated a reallocation of resources from DNA binding to DNA repair and from motility to chemo-taxis and sensing. Changes to iron and nitrogen metabolism, cellular membrane structures, and protein synthesis and folding were also revealed. By elucidating vital strategies during life in ice, this study provides novel insight into the extensive molecular adaptations that occur in cold-adapted marine organisms to sustain cellular function in their habitat. Raw data available at http://www.chorusproject.org Public project ID number 682.
HostingRepository	PRIDE
AnnounceDate	2017-04-13
AnnouncementXML	Submission_2017-04-13_06:36:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brook Nunn
SpeciesList	scientific name: Colwellia psychrerythraea 34H; NCBI TaxID: 167879;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-10 01:53:44	ID requested
⏵ 1	2017-04-13 06:36:25	announced

Nunn BL, Slattery KV, Cameron KA, Timmins-Schiffman E, Junge K, Proteomics of Colwellia psychrerythraea at subzero temperatures - a life with limited movement, flexible membranes and vital DNA repair. Environ Microbiol, 17(7):2319-35(2015) [pubmed]

curator keyword: Biological

submitter keyword: Colwellia pschrerythraea, Adaptation Physiological/genetics, Alteromonadaceae/genetics, Alteromonadaceae/metabolism, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Cold Temperature, DNA Repair, Iron/metabolism, Movement, Nitrogen/metabolism, proteomics

Brook L. Nunn
contact affiliation	University of Washington, Department of Genome Sciences
contact email	brookh@uw.edu
lab head
Brook Nunn
contact affiliation	University of Washington
contact email	brookh@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006274
     1. Label: PRIDE project
     2. Name: Proteomics of Colwellia psychrerythraea grown at multiple subzero temperatures