PXD006271

PXD006271 is an original dataset announced via ProteomeXchange.

Title	Proteome Analysis of Human Embryonic Stem Cells Organelles
Description	Here, we have utilized a sub-cellular proteomics approach to analyze the localization of proteins in the nucleus, mitochondria, crude membrane, cytoplasm, heavy and light microsomes. Out of 2002 reproducibly identified proteins, we detected 762 proteins in a single organelle whereas 160 proteins were found in all sub-cellular fractions. We verified the localization of identified proteins through databases and discussed the consistency of the obtained results. With regards to the ambiguity in the definition of a membrane protein, we tried to clearly define the plasma membrane, peripheral membrane and membrane proteins by annotation of these proteins in databases, along with predictions of transmembrane helices.
HostingRepository	PRIDE
AnnounceDate	2018-02-23
AnnouncementXML	Submission_2018-02-23_00:30:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Faezeh Shekari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-07 01:51:59	ID requested
1	2017-05-02 05:00:12	announced
2	2018-02-14 02:01:04	announced	Updated project metadata.
⏵ 3	2018-02-23 00:30:48	announced	Updated project metadata.

Shekari F, Nezari H, Larijani MR, Han CL, Baharvand H, Chen YJ, Salekdeh GH, Proteome analysis of human embryonic stem cells organelles. J Proteomics, 162():108-118(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human Embryonic Stem Cells, Organelles

Ghasem Hosseini Salekdeh
contact affiliation	Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
contact email	h_salekdeh@abrii.ac.ir
lab head
Faezeh Shekari
contact affiliation	Royan institute
contact email	faezehshekari@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD006271

PRIDE project URI

• PRIDE
  1. PXD006271
     1. Label: PRIDE project
     2. Name: Proteome Analysis of Human Embryonic Stem Cells Organelles