PXD006270

PXD006270 is an original dataset announced via ProteomeXchange.

Title	Profiling the level of N-glycan structural heterogeneity in Arabidopsis N-glycoproteins
Description	In this study, we use hydrophilic enrichment, high-resolution tandem mass spectrometry with complimentary and triggered fragmentation to profile Arabidopsis N-glycopeptides. A total of 492 N-glycosites were identified from 324 Arabidopsis proteins with extensive N-glycan structural heterogeneity revealed through 1099 N-glycopeptides. To demonstrate the precision of the approach, we also profiled N-glycopeptides from the β-1,2-xylosyltransferase mutant (xylt), an enzyme in the N-glycan biosynthetic pathway.
HostingRepository	PRIDE
AnnounceDate	2017-12-18
AnnouncementXML	Submission_2017-12-18_01:53:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kris Ford
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	complex glycosylation; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-07 01:48:00	ID requested
⏵ 1	2017-12-18 01:53:23	announced

Zeng W, Ford KL, Bacic A, Heazlewood JL, -linked Glycan Micro-heterogeneity in Glycoproteins of Arabidopsis. Mol Cell Proteomics, 17(3):413-421(2018) [pubmed]

curator keyword: Biological

submitter keyword: N-glycosylation, Arabidopsis, glycopeptides

Joshua L. Heazlewood
contact affiliation	1. ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia 2. Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94702, USA
contact email	jheazlewood@unimelb.edu.au
lab head
Kris Ford
contact affiliation	School of Biosciences
contact email	kferg@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006270

PRIDE project URI

• PRIDE
  1. PXD006270
     1. Label: PRIDE project
     2. Name: Profiling the level of N-glycan structural heterogeneity in Arabidopsis N-glycoproteins