PXD006251

PXD006251 is an original dataset announced via ProteomeXchange.

Title	Ndfip1 restricts mTORC1 signalling and glycolysis in regulatory T cells to prevent autoinflammatory disease
Description	Project abstract: Foxp3+ T regulatory (Treg) cells have important functions in suppressing immune cell activation and establishing normal immune homeostasis. How Treg cells maintain their identity is not completely understood. Here we show that Ndfip1, a co-activator of Nedd4-family E3 ubiquitin ligases, is required for Treg cell stability and function. Ndfip1 deletion in Treg cells disrupts immune homeostasis and results in autoinflammatory disease. Ndfip1-deficient Treg cells are highly proliferative and are more likely to lose Foxp3 expression to become IL-4-producing TH2 effector cells. Proteomic analyses indicate that Ndfip1 deficiency alters the metabolic signature of Treg cells. Metabolic profiling reveals elevated glycolysis and increased mTORC1 signalling. Additional data suggest that Ndfip1 restricts Treg cell metabolic capacity and IL-4 production via distinct mechanisms. Thus, Ndfip1 preserves Treg lineage stability by preventing the expansion of highly proliferative and metabolically active cells that can cause immunopathology via secretion of IL-4.
HostingRepository	PRIDE
AnnounceDate	2017-04-19
AnnouncementXML	Submission_2019-02-12_08:19:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Seeholzer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-04 01:20:28	ID requested
1	2017-04-19 03:14:24	announced
⏵ 2	2019-02-12 08:19:12	announced	Updated publication reference for PubMed record(s): 28580955.

Layman AAK, Deng G, O'Leary CE, Tadros S, Thomas RM, Dybas JM, Moser EK, Wells AD, Doliba NM, Oliver PM, Ndfip1 restricts mTORC1 signalling and glycolysis in regulatory T cells to prevent autoinflammatory disease. Nat Commun, 8():15677(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Ndfip1, Regulatory T cells (Treg cells), effector-type Regulatory T cells (eTreg cells), central Regulatory T cells (cTreg cells)

Paula Oliver
contact affiliation	Cell Pathology Division 816F/ARC Children’s Hospital of Philadelphia The University of Pennsylvania 3615 Civic Center Blvd. 816F/ARC Philadelphia, PA 19104
contact email	paulao@mail.med.upenn.edu
lab head
Steven Seeholzer
contact affiliation	Children's Hospital of Philadelphia
contact email	seeholzer@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD006251

PRIDE project URI

• PRIDE
  1. PXD006251
     1. Label: PRIDE project
     2. Name: Ndfip1 restricts mTORC1 signalling and glycolysis in regulatory T cells to prevent autoinflammatory disease