PXD006250

PXD006250 is an original dataset announced via ProteomeXchange.

Title	Surviving oxidative stress under iron starvation: an important lesson eminently learned by Aspergillus fumigatus in the human body
Description	Aspergillus fumigatus has to cope with a combination of several stress types after colonisng the human body, and the interplay between the different stress responses can significantly influence the survival of this human pathogen during the invasion of the host organism. In this study, we examined how the H2O2 induced oxidative stress response depends on iron availability. Surprisingly, the applied H2O2 treatment, which induced only a negligible stress response in iron fed cultures, deleteriously affected the fungus under iron starvation and the majority of stress responses on the transcript level were characteristic only for the combined H2O2-iron starvation stress treatments. Our data suggest that the survival of the fungus highly depends on fragile balances, e.g. between siderophore and ergosterol productions or between economization on iron and production of essential iron containing proteins. The applied stress conditions also affected several processes related to virulence or drug susceptibility including secondary metabolism, zinc acquisition or antifungal drug transport. Our[It would be interesting to mention the antioxidative enzymes which are upregulated/increase in abundance under iron-depleting conditions] data clearly demonstrate that studying stress responses under single stress treatments is not sufficient to understand how fungal pathogens survive in a complex habitat and support the view that the evolutionary success of A. fumigatus as an opportunistic human pathogen is not the mere consequence of the productions of certain virulence factors. Importantly, this fungal pathogen is able to mount and coordinate high-complexity and outstandingly efficient responses to multiple and superpositioning stresses in various harsh habitats like the human body.
HostingRepository	PRIDE
AnnounceDate	2018-05-17
AnnouncementXML	Submission_2018-05-17_07:47:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Aspergillus fumigatus Af293; NCBI TaxID: 330879;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-04 00:45:30	ID requested
1	2018-05-16 04:08:59	announced
⏵ 2	2018-05-17 07:47:44	announced	Updated project metadata.

Kurucz V, Kr, ü, ger T, Antal K, Dietl AM, Haas H, P, ó, csi I, Kniemeyer O, Emri T, Additional oxidative stress reroutes the global response of Aspergillus fumigatus to iron depletion. BMC Genomics, 19(1):357(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Aspergillus fumigatus, oxidative stress, iron starvation, stress response, proteomics, tandem mass tags, LC-MS

Olaf Kniemeyer
contact affiliation	Stress- and Immunoproteomics group, Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena, Germany
contact email	olaf.kniemeyer@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD006250

PRIDE project URI

• PRIDE
  1. PXD006250
     1. Label: PRIDE project
     2. Name: Surviving oxidative stress under iron starvation: an important lesson eminently learned by Aspergillus fumigatus in the human body