PXD006247

PXD006247 is an original dataset announced via ProteomeXchange.

Title	Global Landscape of Cell Envelope Protein Complexes in Escherichia coli
Description	After extraction with mild non-denaturing detergents, we affinity-purified 785 endogenously-tagged CEPs and then identified stably-associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising most (77%) of all targeted CEPs, revealed hundreds of previously unknown heteromeric complexes. Lab Heads: Andrew Emili; andrew.emili@utoronto.ca ;Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada Mohan Babu; mohan.babu@uregina.ca ;Research and Innovation Centre, University of Regina, SK S4S OA2, Canada
HostingRepository	PRIDE
AnnounceDate	2017-12-01
AnnouncementXML	Submission_2017-12-11_04:06:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sadhna Phanse
SpeciesList	scientific name: Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911); NCBI TaxID: 316407;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-03 08:07:53	ID requested
1	2017-11-30 18:22:12	announced
⏵ 2	2017-12-11 04:07:02	announced	Updated publication reference for PubMed record(s): 29176613.

Babu M, Bundalovic-Torma C, Calmettes C, Phanse S, Zhang Q, Jiang Y, Minic Z, Kim S, Mehla J, Gagarinova A, Rodionova I, Kumar A, Guo H, Kagan O, Pogoutse O, Aoki H, Deineko V, Caufield JH, Holtzapple E, Zhang Z, Vastermark A, Pandya Y, Lai CC, El Bakkouri M, Hooda Y, Shah M, Burnside D, Hooshyar M, Vlasblom J, Rajagopala SV, Golshani A, Wuchty S, F Greenblatt J, Saier M, Uetz P, F Moraes T, Parkinson J, Emili A, Global landscape of cell envelope protein complexes in Escherichia coli. Nat Biotechnol, 36(1):103-112(2018) [pubmed]

curator keyword: Biological

submitter keyword: Cell Envelope, Cell envelope proteins, Cell envelope Protein complexes, Escherichia coli, Macromolecues, Multi-protein complexes, Protein-protein interactions

Andrew Emili
contact affiliation	Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada
contact email	andrew.emili@utoronto.ca
lab head
Sadhna Phanse
contact affiliation	University of Toronto
contact email	sadhna.phanse@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006247

PRIDE project URI

• PRIDE
  1. PXD006247
     1. Label: PRIDE project
     2. Name: Global Landscape of Cell Envelope Protein Complexes in Escherichia coli