PXD006242

PXD006242 is an original dataset announced via ProteomeXchange.

Title	Dietary effect on the proteome of the common octopus (Octopus vulgaris) paralarvae, 2D-DIGE gel data
Description	Nowadays, the common octopus (Octopus vulgaris) culture is hampered by massive mortalities occurring during early life-cycle stages (paralarvae). Despite the causes of the high paralarvae mortality are not yet well defined and understood, the nutritional stress caused by inadequate diets is pointed out as one of the main factors. In this study the effects of diet on paralarvae is analyzed through a proteomic approach, to search for novel biomarkers of nutritional stress. A total of 43 proteins showing differential expression in the different conditions studied have been identified. The analysis highlights proteins related with the carbohydrate metabolism: glyceraldehyde-3-phosphate-dedydrogenase (GAPDH), triosephosphate isomerase; other ways of energetic metabolism: NADP+-specific isocitrate dehydrogenase, arginine kinase; detoxification: glutathione-S-transferase (GST); stress: heat shock proteins (HSP70); structural constituent of eye lens: S-crystallin 3; and cytoskeleton: actin, actin-beta/gamma1, beta actin. These results allow defining characteristic proteomes of paralarvae depending on the diet; as well as the use of several of these proteins as novel biomarkers to evaluate their welfare linked to nutritional stress. Notably, the changes of proteins like S-crystallin 3, arginine kinase and NAD+ specific isocitrate dehydrogenase, may be related to fed versus starving paralarvae, particularly in the first 4 days of development.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:10.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Octopus vulgaris; NCBI TaxID: 6645;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-03 05:12:54	ID requested
1	2022-03-01 09:58:21	announced
⏵ 2	2023-11-14 08:49:10	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: nutritional stress,Octopus vulgaris, welfare, paralarvae, proteome, novel biomarkers

Inmaculada Varo
contact affiliation	Instituto de Acuicultura Torre de la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
contact email	inma@iats.csic.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD006242

PRIDE project URI

• PRIDE
  1. PXD006242
     1. Label: PRIDE project
     2. Name: Dietary effect on the proteome of the common octopus (Octopus vulgaris) paralarvae, 2D-DIGE gel data