PXD006240 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry identification of proteins of isolated auditory and vestibular hair cells using data-independent and data-dependent acquisition |
Description | Hair cells undergo postnatal development that leads to formation of their sensory organelles, synaptic machinery, and in the case of cochlear outer hair cells, their electromotile mechanism. To examine the proteome changes over development, we isolated pools of 5000 Pou4f3-Gfp positive or negative cells from the cochlea or utricles; these cell pools were analyzed by data-dependent and data-independent acquisition (DDA and DIA) mass spectrometry. DDA data were used to generate spectral libraries, which enabled identification and accurate quantitation of specific proteins using the DIA datasets. We also isolated and pooled individual inner and outer hair cells from adult cochlea and compared their proteomes to those of developing hair cells. The DDA and DIA datasets will be valuable for accurately quantifying proteins in hair cells and non-hair cells over this developmental window. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:02:18.790.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Barr-Gillespie |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-03 03:55:38 | ID requested | |
1 | 2017-09-18 00:13:55 | announced | |
⏵ 2 | 2024-10-22 04:02:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
Avenarius MR, Krey JF, Dumont RA, Morgan CP, Benson CB, Vijayakumar S, Cunningham CL, Scheffer DI, Corey DP, M, ΓΌ, ller U, Jones SM, Barr-Gillespie PG, Heterodimeric capping protein is required for stereocilia length and width regulation. J Cell Biol, 216(11):3861-3881(2017) [pubmed] |
10.1083/jcb.201704171; |
Keyword List
curator keyword: Biological |
submitter keyword: supporting cells, hair cells, cochlea,Utricle |
Contact List
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 |
contact email | gillespp@ohsu.edu |
lab head | |
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center |
contact email | gillespp@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006240 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006240
- Label: PRIDE project
- Name: Mass spectrometry identification of proteins of isolated auditory and vestibular hair cells using data-independent and data-dependent acquisition