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PXD006240

PXD006240 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry identification of proteins of isolated auditory and vestibular hair cells using data-independent and data-dependent acquisition
DescriptionHair cells undergo postnatal development that leads to formation of their sensory organelles, synaptic machinery, and in the case of cochlear outer hair cells, their electromotile mechanism. To examine the proteome changes over development, we isolated pools of 5000 Pou4f3-Gfp positive or negative cells from the cochlea or utricles; these cell pools were analyzed by data-dependent and data-independent acquisition (DDA and DIA) mass spectrometry. DDA data were used to generate spectral libraries, which enabled identification and accurate quantitation of specific proteins using the DIA datasets. We also isolated and pooled individual inner and outer hair cells from adult cochlea and compared their proteomes to those of developing hair cells. The DDA and DIA datasets will be valuable for accurately quantifying proteins in hair cells and non-hair cells over this developmental window.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:02:18.790.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeter Barr-Gillespie
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-03 03:55:38ID requested
12017-09-18 00:13:55announced
22024-10-22 04:02:19announced2024-10-22: Updated project metadata.
Publication List
Avenarius MR, Krey JF, Dumont RA, Morgan CP, Benson CB, Vijayakumar S, Cunningham CL, Scheffer DI, Corey DP, M, ΓΌ, ller U, Jones SM, Barr-Gillespie PG, Heterodimeric capping protein is required for stereocilia length and width regulation. J Cell Biol, 216(11):3861-3881(2017) [pubmed]
10.1083/jcb.201704171;
Keyword List
curator keyword: Biological
submitter keyword: supporting cells, hair cells, cochlea,Utricle
Contact List
Peter Barr-Gillespie
contact affiliationOregon Hearing Research Center Oregon Health & Science University Portland OR 97239
contact emailgillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliationOregon Hearing Research Center
contact emailgillespp@ohsu.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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