PXD006240

PXD006240 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry identification of proteins of isolated auditory and vestibular hair cells using data-independent and data-dependent acquisition
Description	Hair cells undergo postnatal development that leads to formation of their sensory organelles, synaptic machinery, and in the case of cochlear outer hair cells, their electromotile mechanism. To examine the proteome changes over development, we isolated pools of 5000 Pou4f3-Gfp positive or negative cells from the cochlea or utricles; these cell pools were analyzed by data-dependent and data-independent acquisition (DDA and DIA) mass spectrometry. DDA data were used to generate spectral libraries, which enabled identification and accurate quantitation of specific proteins using the DIA datasets. We also isolated and pooled individual inner and outer hair cells from adult cochlea and compared their proteomes to those of developing hair cells. The DDA and DIA datasets will be valuable for accurately quantifying proteins in hair cells and non-hair cells over this developmental window.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:02:18.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barr-Gillespie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-03 03:55:38	ID requested
1	2017-09-18 00:13:55	announced
⏵ 2	2024-10-22 04:02:19	announced	2024-10-22: Updated project metadata.

Avenarius MR, Krey JF, Dumont RA, Morgan CP, Benson CB, Vijayakumar S, Cunningham CL, Scheffer DI, Corey DP, M, ü, ller U, Jones SM, Barr-Gillespie PG, Heterodimeric capping protein is required for stereocilia length and width regulation. J Cell Biol, 216(11):3861-3881(2017) [pubmed]

10.1083/jcb.201704171;

curator keyword: Biological

submitter keyword: supporting cells, hair cells, cochlea,Utricle

Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239
contact email	gillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center
contact email	gillespp@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006240

PRIDE project URI

• PRIDE
  1. PXD006240
     1. Label: PRIDE project
     2. Name: Mass spectrometry identification of proteins of isolated auditory and vestibular hair cells using data-independent and data-dependent acquisition