PXD006229
PXD006229 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency |
| Description | Chromatin-associated fumarase (FH) affects histone methylation via its metabolic activity. However, whether this regulation is implicated in gene transcription remains to be clarified. In this study, we show that glucose deprivation induces AMPK-dependent phosphorylation of FH at Ser 75, which promotes association of FH with transcriptional factor ATF2 and related gene promoters. The inhibitory effect of local fumarate on KDM2A histone demethylase maintains H3K36me2, thus facilitates transcription for cell growth arrest. On the other hand, FH is found to be O-GlcNAcylated at the AMPK phosphorylation site; FH-ATF2-mediated downstream events are impeded by FH O-GlcNAcylation especially in cancer cells that display the robust OGT activity. Consistently, FH-S75 phosphorylation levels inversely correlate with the OGT level and poor prognosis in pancreatic cancer patients. These findings uncover a mechanism underlying transcription regulation by metabolic enzyme and the linkage between dysregulated OGT activity and growth advantage of cancer cells under nutrient stress. |
| HostingRepository | MassIVE |
| AnnounceDate | 2017-04-01 |
| AnnouncementXML | Submission_2017-04-01_04:28:42.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Houqin |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho; O-(N-acetylamino)glucosyl-L-serine |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-04-01 03:58:10 | ID requested | |
| ⏵ 1 | 2017-04-01 04:28:42 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: fumarase, phosphorylation, O-GlcNAcylation |
Contact List
| Yuhui Jiang | |
|---|---|
| contact affiliation | The Institute of Cell Metabolism, Shanghai Key Laboratory of Pancreatic Disease, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine |
| contact email | oxfordman@hotmail.com |
| lab head | |
| Houqin | |
| contact affiliation | ECNU |
| contact email | 1160663589@qq.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080872 |
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