PXD006226

PXD006226 is an original dataset announced via ProteomeXchange.

Title	The HP1 protein interactome of mouse embryonic stem cells
Description	The family of Heterochromatin Protein 1 (HP1) consists of highly conserved proteins, which have important functions in the nucleus of eukaryotic cells. In mammals there are three HP1 paralogs: HP1(alpha), Hp1(beta), and Hp1(gamma)They are encoded by the Cbx5, Cbx1, and Cbx3 genes, respectively. Hp1 and Hp1 stably interact with Chd4 and Adnp to form the ChAHP complex. In this project, Chd4, Adnp, and the three Cbx genes were endogenously tagged with a FLAG-Avi tag in mouse embryonic stem cells. The tagged proteins were subjected to tandem-affinity purification and analysis by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2018-05-22
AnnouncementXML	Submission_2018-05-30_08:14:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vytautas Iesmantavicius
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-31 08:05:12	ID requested
1	2018-05-22 06:41:46	announced
⏵ 2	2018-05-30 08:14:58	announced	Updated publication reference for PubMed record(s): 29795351.

Ostapcuk V, Mohn F, Carl SH, Basters A, Hess D, Iesmantavicius V, Lampersberger L, Flemr M, Pandey A, Thom, ä NH, Betschinger J, B, ü, hler M, Activity-dependent neuroprotective protein recruits HP1 and CHD4 to control lineage-specifying genes. Nature, 557(7707):739-743(2018) [pubmed]

curator keyword: Biological

submitter keyword: Adnp, Chd4, HP1, Hp1, Hp1, Cbx1, Cbx3, Cbx5, mouse ES-cells, FLAG-Avi-tag, tandem purification, LC-Orbitrap Fusion, MaxQuant.

Marc Bühler
contact affiliation	Group Leader in Epigenetics, Friedrich Miescher Institute, Basel, Switzerland
contact email	Marc.Buehler@fmi.ch
lab head
Vytautas Iesmantavicius
contact affiliation	Friedrich Miescher Institute for Biomedical Research
contact email	vytautas.iesmantavicius@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD006226

PRIDE project URI

• PRIDE
  1. PXD006226
     1. Label: PRIDE project
     2. Name: The HP1 protein interactome of mouse embryonic stem cells