PXD006206

PXD006206 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MudPIT analyses of the human proteins biotinylated by proximity to dynein/dynactin subunits fused to biotin ligase (BioID)
Description	We identified the human dynein proteome by attaching a promiscuous biotin ligase (BioID) to 7 distinct components of the dynein machinery, including a subunit of its essential cofactor dynactin. BioID experiments were performed with stable HEK293 cell lines expressing dynein (IC1, IC2, LIC1, LIC2, RB, TcTex-1) and dynactin (p62) subunits tagged with BioID-3X FLAG. BioID-only transfected cells were used as negative controls. For BioID experiments, cells were lysed in the presence of detergents to disrupt the dynein/dynactin complex, allowing the identification of proteins that were proximal to the tagged subunit prior to cell lysis. After purification of biotinylated proteins on streptavidin-conjugated beads, the eluates were precipitated with TCA. After washing with acetone, the protein mixtures were digested with endoproteinase Lys-C and trypsin (Roche) and analyzed by MudPIT. We performed BioID purification followed by MudPIT in quadruplicate and used a label-free quantitative proteomics approach to calculate the enrichment of each identified protein relative to BioID control replicates.
HostingRepository	MassIVE
AnnounceDate	2017-07-31
AnnouncementXML	Submission_2017-07-31_06:36:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurence Florens
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-29 09:59:49	ID requested
⏵ 1	2017-07-31 06:36:40	announced

Publication List

Redwine WB, DeSantis ME, Hollyer I, Htet ZM, Tran PT, Swanson SK, Florens L, Washburn MP, Reck-Peterson SL, The human cytoplasmic dynein interactome reveals novel activators of motility. Elife, 6():(2017) [pubmed]

Redwine WB, DeSantis ME, Hollyer I, Htet ZM, Tran PT, Swanson SK, Florens L, Washburn MP, Reck-Peterson SL, The human cytoplasmic dynein interactome reveals novel activators of motility. Elife, 6():(2017) [pubmed]

Keyword List

submitter keyword: Cytoplasmic dynein-1, dynactin, activator, promiscuous biotin ligase

submitter keyword: Cytoplasmic dynein-1, dynactin, activator, promiscuous biotin ligase

Contact List

Laurence Florens
contact affiliation	The Stowers Institute for Medical Research
contact email	laf@stowers.org
lab head
Laurence Florens
contact affiliation	Stowers Institute for Medical Research
contact email	laf@stowers.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080759