PXD006199 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | R2TP_SILAC_FLAG-MS |
Description | The R2TP/Prefoldin-like (R2TP/PFDL) complex is a cochaperone complex involved in the assembly of a number of critical protein complexes. Here we use stable isotope labeling with amino acids in cell culture (SILAC) and FLAG tag affinity purification coupled to mass spectrometry to assess whether siRNA-driven knockdown of cofactors or subunits of R2TP/PFDN affects composition of U5 snRNP and TSC complex. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-02 |
AnnouncementXML | Submission_2017-11-02_04:46:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Poitras |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-29 01:33:07 | ID requested | |
⏵ 1 | 2017-11-02 04:46:07 | announced | |
Publication List
Cloutier P, Poitras C, Durand M, Hekmat O, Fiola-Masson É, Bouchard A, Faubert D, Chabot B, Coulombe B, R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun, 8():15615(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, HEK 293, FLAG, affinity purification, LC-MSMS, ZNHIT2, U5 snRNP, TSC complex |
Contact List
Benoit Coulombe |
contact affiliation | Institut de recherches cliniques de Montréal (IRCM), Translational proteomics |
contact email | benoit.coulombe@ircm.qc.ca |
lab head | |
Christian Poitras |
contact affiliation | Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada |
contact email | christian.poitras@ircm.qc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006199 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006199
- Label: PRIDE project
- Name: R2TP_SILAC_FLAG-MS