Updated publication reference for PubMed record(s): 29738528. We utilized high resolution, high mass accuracy quantitative proteomics to explore stress signaling in yeast. We accessed changes in protein phosphorylation at various time points after exposure to salt stress and used this information to reconstruct stress signaling networks. We performed similar experiments using yeast knockouts to monitor network re-wiring and performed co-IPs to validate protein-protein interactions predicted by the networks.