PXD006186

PXD006186 is an original dataset announced via ProteomeXchange.

Title	High-resolution mass spectrometry based label-free quantification of proteome changes during bile salt exposure of Lactobacillus fermentum RS2
Description	Lactobacillus fermentum (strain RS2) is a common resident of the human gastrointestinal tract (GIT) and used as potential probiotics. The ability to adapt, colonize and survive in the harsh environment of the GIT, in the presence of gastric juice, is one of the unique properties that helps microflora to transiently harbor the host. In the current study, we used gel free quantification approach coupled with a high-resolution mass spectrometer to analyze the expression pattern of Lactobacillus fermentum RS2 strain under 1.2% bile acid stress condition. The analysis resulted in the identification of 1157 varieties of proteins that are engaged in coping up the efflux of bile effect or protons, by altering the carbohydrate metabolic pathways or through prevention of protein misfolding. The function of downregulated proteins were mostly unknown and the putative functions of the upregulated proteins were categorized as stress response, DNA repair, amino acids metabolism, signal transduction, transcription, translation, and carbohydrate metabolism. The finding suggests that these proteins are involved in bile resistance specific mechanisms as well as processes responsible for adaptation in GIT and showed the association of expression levels of proteins with a precise probiotic trait.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_02:57:56.511.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pradip Behare
SpeciesList	scientific name: Lactobacillus fermentum CECT 5716; NCBI TaxID: 712938;
ModificationList	carboxylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-27 07:51:32	ID requested
⏵ 1	2022-03-02 02:57:56	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Probiotics, Lactobacillus fermentum, High-resolution mass spectrometer, Cytoscape, DAVID

Dr. Pradip Behare
contact affiliation	Dr. Pradip Behare Scientist- Department of Microbiology National Dairy Research Institute Karnal - 132001 Haryana, India
contact email	pradip_behare@yahoo.com
lab head
Pradip Behare
contact affiliation	National Dairy Research Institute
contact email	pradip_behare@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006186
     1. Label: PRIDE project
     2. Name: High-resolution mass spectrometry based label-free quantification of proteome changes during bile salt exposure of Lactobacillus fermentum RS2