PXD006185

PXD006185 is an original dataset announced via ProteomeXchange.

Title	Hsp90-downregulation influences the heat-shock response, innate immune response and onset of oocyte development in nematodes.
Description	Hsp90 is a molecular chaperone involved in the regulation and maturation of kinases and transcription factors. In C. elegans Hsp90 participates in the development of fertility, maintenance of muscle structure and the regulation of stress response and dauer state. To understand the consequences of Hsp90-depletion we studied Hsp90 RNAi-treated nematodes by DNA microarrays and mass spectrometry. We find that upon development of phenotypes the levels of chaperones and cofactors are increased, while specific proteins related to the innate immune response are specifically depleted. We further uncover Hsp90-dependent signatures of gonad development and larval development. Using reporter strains for the responsive genes skr-5, dod-24 and clec-60, we confirm an influence on the innate immune response in intestinal tissues, while alterations to development become evident in strains reporting on the presence of ZIP-8, CAV-1 and SEPA-1 in the gonad arms and very early embryos. These observations add molecular details to the Hsp90-dependent processes during development and stress responses and imply that multiple interactions of this chaperone system are relevant for the nematode’s life and development.
HostingRepository	PRIDE
AnnounceDate	2018-10-17
AnnouncementXML	Submission_2018-10-17_01:25:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006185
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Claudia Lindemann
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-27 06:51:08	ID requested
⏵ 1	2018-10-17 01:25:04	announced

Eckl J, Sima S, Marcus K, Lindemann C, Richter K, Hsp90-downregulation influences the heat-shock response, innate immune response and onset of oocyte development in nematodes. PLoS One, 12(10):e0186386(2017) [pubmed]

curator keyword: Biological

submitter keyword: Hsp90, immune system, LC/MS, nematodes

Prof. Dr. Katrin Marcus
contact affiliation	Ruhr-Universität Bochum Zentrum für klinische Forschung (ZKF) Room 1.055 Universitätsstraße 150 44801 Bochum, Germany
contact email	katrin.marcus@rub.de
lab head
Claudia Lindemann
contact affiliation	Medizinisches Proteom-Center, Ruhr Universität Bochum
contact email	claudia.lindemann@rub.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006185
     1. Label: PRIDE project
     2. Name: Hsp90-downregulation influences the heat-shock response, innate immune response and onset of oocyte development in nematodes.