PXD006183 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of the short chain fatty acid, sodium butyrate, on gut resident microbiota, gut mucus proteome and fish susceptibility to bacterial and parasitic infections in vivo |
Description | Previous works in the framework of EU ARRAINA Project evidenced a pro-inflammatory condition in gilthead sea bream (Sparus aurata) fed extremely low fish meal/fish oil diets, and this effect was mostly reversed by butyrate supplementation. The hypothesis of work is that these nutritionally-mediated changes can be extensive to intestinal mucus proteome and gut microbiota, which in turn could modify disease outcome.s If so, the prevalence and progression of the disease might be also modified by diet composition and feed additives. Gilthead sea bream fingerlings were fed with control and experimental diets formulated by BioMar until two year-old. FM was added at 25% in the control diet (D1) and at 5% in the other three diets (D2-D4). Added oil was either FO (D1 control diet) or a blend of vegetable oils, replacing the 58% (D2) and the 84% (D3-D4 diets) of FO. A commercial sodium butyrate preparation (NOREL, BP70) was added to the D4 diet at 0.4%. At month 20, 6 fish per each dietary treatment were sampled for iTRAQ profiling and fingerprinting of intestinal mucus proteome. Mucus collected from anterior and posterior intestine segments was trypsin digested, labelled with iTRAQ reagents, isoelectrofocused and resolved by LC-MS/MS. More than 1000 proteins were unequivocally annotated and principal component analysis clearly separated anterior and posterior segments. The diet effect with changes in the abundance of approximately 120 proteins was restricted to anterior section with a reversion of the pattern of the extreme diet (D3 fish) with dietary butyrate supplementation. Butyrate supplementation also reversed the decrease of microbiotay diversity associated with D3 feeding, and led to a improvement the disease outcomes in fish challenged with Photobacterium damselae and the intestinal parasite Enteromyxum leei. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:05:14.624.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luz Valero |
SpeciesList | scientific name: Sparus aurata; NCBI TaxID: 8175; |
ModificationList | iTRAQ8plex-113 reporter+balance reagent N6-acylated lysine; monohydroxylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-27 05:42:55 | ID requested | |
1 | 2018-10-24 14:47:10 | announced | |
⏵ 2 | 2024-10-22 04:05:20 | announced | 2024-10-22: Updated project metadata. |
Publication List
Piazzon MC, Calduch-Giner JA, Fouz B, Estensoro I, Sim, ó, -Mirabet P, Puyalto M, Karalazos V, Palenzuela O, Sitj, à, -Bobadilla A, P, é, rez-S, á, nchez J, Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets. Microbiome, 5(1):164(2017) [pubmed] |
10.1186/s40168-017-0390-3; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: gut |
Microbiome |
Proteome |
Disease resilience,Fish meal, Butyrate, Fish oil |
Contact List
Jaume Pérez-Sánchez |
contact affiliation | Nutrigenomics and Fish Growth Endocrinology Group, Institute of Aquaculture Torre de la Sal, Castellón, Spain |
contact email | jaime.perez.sanchez@csic.es |
lab head | |
Luz Valero |
contact affiliation | Universitat de Valencia |
contact email | mluz.valero@uv.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006183
- Label: PRIDE project
- Name: Impact of the short chain fatty acid, sodium butyrate, on gut resident microbiota, gut mucus proteome and fish susceptibility to bacterial and parasitic infections in vivo