PXD006183

PXD006183 is an original dataset announced via ProteomeXchange.

Title	Impact of the short chain fatty acid, sodium butyrate, on gut resident microbiota, gut mucus proteome and fish susceptibility to bacterial and parasitic infections in vivo
Description	Previous works in the framework of EU ARRAINA Project evidenced a pro-inflammatory condition in gilthead sea bream (Sparus aurata) fed extremely low fish meal/fish oil diets, and this effect was mostly reversed by butyrate supplementation. The hypothesis of work is that these nutritionally-mediated changes can be extensive to intestinal mucus proteome and gut microbiota, which in turn could modify disease outcome.s If so, the prevalence and progression of the disease might be also modified by diet composition and feed additives. Gilthead sea bream fingerlings were fed with control and experimental diets formulated by BioMar until two year-old. FM was added at 25% in the control diet (D1) and at 5% in the other three diets (D2-D4). Added oil was either FO (D1 control diet) or a blend of vegetable oils, replacing the 58% (D2) and the 84% (D3-D4 diets) of FO. A commercial sodium butyrate preparation (NOREL, BP70) was added to the D4 diet at 0.4%. At month 20, 6 fish per each dietary treatment were sampled for iTRAQ profiling and fingerprinting of intestinal mucus proteome. Mucus collected from anterior and posterior intestine segments was trypsin digested, labelled with iTRAQ reagents, isoelectrofocused and resolved by LC-MS/MS. More than 1000 proteins were unequivocally annotated and principal component analysis clearly separated anterior and posterior segments. The diet effect with changes in the abundance of approximately 120 proteins was restricted to anterior section with a reversion of the pattern of the extreme diet (D3 fish) with dietary butyrate supplementation. Butyrate supplementation also reversed the decrease of microbiotay diversity associated with D3 feeding, and led to a improvement the disease outcomes in fish challenged with Photobacterium damselae and the intestinal parasite Enteromyxum leei.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:14.624.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Sparus aurata; NCBI TaxID: 8175;
ModificationList	iTRAQ8plex-113 reporter+balance reagent N6-acylated lysine; monohydroxylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-27 05:42:55	ID requested
1	2018-10-24 14:47:10	announced
⏵ 2	2024-10-22 04:05:20	announced	2024-10-22: Updated project metadata.

Piazzon MC, Calduch-Giner JA, Fouz B, Estensoro I, Sim, ó, -Mirabet P, Puyalto M, Karalazos V, Palenzuela O, Sitj, à, -Bobadilla A, P, é, rez-S, á, nchez J, Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets. Microbiome, 5(1):164(2017) [pubmed]

10.1186/s40168-017-0390-3;

curator keyword: Biological, Biomedical

submitter keyword: gut

Microbiome

Proteome

Disease resilience,Fish meal, Butyrate, Fish oil

Jaume Pérez-Sánchez
contact affiliation	Nutrigenomics and Fish Growth Endocrinology Group, Institute of Aquaculture Torre de la Sal, Castellón, Spain
contact email	jaime.perez.sanchez@csic.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006183

PRIDE project URI

• PRIDE
  1. PXD006183
     1. Label: PRIDE project
     2. Name: Impact of the short chain fatty acid, sodium butyrate, on gut resident microbiota, gut mucus proteome and fish susceptibility to bacterial and parasitic infections in vivo