PXD006182

PXD006182 is an original dataset announced via ProteomeXchange.

Title	Single muscle fiber proteomics reveals fiber type-specific features of human muscle aging
Description	Skeletal muscle is a key tissue in human aging, which affects different muscle fiber types unequally. We developed a highly sensitive single muscle fiber proteomics workflow to study human aging and show that the senescence of slow and fast muscle fibers is characterized by diverging metabolic and protein quality control adaptations. Whereas mitochondrial content declines with aging in both fiber types, glycolysis and glycogen metabolism are upregulated in slow but downregulated in fast muscle fibers. Aging mitochondria decrease expression of the redox enzyme monoamine oxidase A. Slow fibers upregulate a subset of actin and myosin chaperones, whereas an opposite change happens in fast fibers. These changes in metabolism and sarcomere quality control may be related to the ability of slow, but not fast, muscle fibers to maintain their mass during aging. We conclude that single muscle fiber analysis by proteomics can elucidate pathophysiology in a sub-type specific manner.
HostingRepository	PRIDE
AnnounceDate	2017-06-14
AnnouncementXML	Submission_2017-06-22_00:34:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marta Murgia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-27 05:04:53	ID requested
1	2017-06-14 04:38:46	announced
⏵ 2	2017-06-22 00:34:50	announced	Updated publication reference for PubMed record(s): 28614723.

Murgia M, Toniolo L, Nagaraj N, Ciciliot S, Vindigni V, Schiaffino S, Reggiani C, Mann M, Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging. Cell Rep, 19(11):2396-2409(2017) [pubmed]

curator keyword: Biological

submitter keyword: Skeletal muscle, aging, muscle fibers

Matthias Mann
contact affiliation	Max-Planck-Institute of Biochemistry, Martinsried 82152, Germany
contact email	mmann@biochem.mpg.de
lab head
Marta Murgia
contact affiliation	MPI Biochemistry
contact email	mmurgia@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006182

PRIDE project URI

• PRIDE
  1. PXD006182
     1. Label: PRIDE project
     2. Name: Single muscle fiber proteomics reveals fiber type-specific features of human muscle aging