PXD006178 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A quantitative proteomic based approach to study the genistein induced epigenetic reprogramming in MDA-MB231 cells |
Description | Epidemiological data indicate that consumption of soy-based food significantly reduces the cancer risk in human through interaction with estrogen receptors and the ‘phytoestrogen’ genistein present in the soy is responsible for this chemopreventive activity. The epigenome regulatory effect of genistein also reported but the key mechanism behind this effect remain elusive. In this current project, we reported the epigenome modulation effect of genistein using MDA-MB231 cells. Cells were treated with low-dose genistein for >1 month and the stable epigenetic alterations were analyzed by partial MNase digestion and TMT-based quantitative proteomics based chromatome mapping approach. We identified a total of 3177 chromatin-bound proteins with high confidence, including 882 proteins that displayed altered binding topology after cell conditioning with genistein. Prolonged phytochemical exposure permanently modified the binding topology of the key epigenetic regulators and preserved their binding topology in untreated progeny. Genistein induced altered epigenome modulation negatively regulates the expression of cell proliferation genes and suppress cell growth. In contrast, previously exposed cells displayed reduced expression of DNA repair genes and enhanced genotoxic stress upon genistein withdrawal. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:05:05.462.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bamaprasad Dutta |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-27 03:33:52 | ID requested | |
1 | 2018-07-05 04:06:28 | announced | |
⏵ 2 | 2024-10-22 04:05:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1002/pmic.201700474; |
Dutta B, Park JE, Qing ITY, Kon OL, Sze SK, Soy-Derived Phytochemical Genistein Modifies Chromatome Topology to Restrict Cancer Cell Proliferation. Proteomics, 18(16):e1700474(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: epigenome, epigenetics, DNA repair, TMT,Genistein, chromatome, heterochromatin, epigenetic reprogram, euchromatin, quantitative proteomics, chromatin |
Contact List
Siu Kwan Sze |
contact affiliation | School of Biological Sciences Division of Structural Biology and Biochemistry Nanyang Technological University, 60 Nanyang drive, Singapore 637551 |
contact email | sksze@ntu.edu.sg |
lab head | |
Bamaprasad Dutta |
contact affiliation | Nanyang Technological University |
contact email | bama0001@e.ntu.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006178
- Label: PRIDE project
- Name: A quantitative proteomic based approach to study the genistein induced epigenetic reprogramming in MDA-MB231 cells