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PXD006166

PXD006166 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTowards a quantitative assessment of the integral magnetosome membrane sub-proteome
DescriptionMagnetosomes are membranous organelles than contain crystals of magnetic minerals and enable magnetotactic bacteria to orientate in magnetic fields. The enveloping magnetosome membrane was found to contain a subset of specific proteins that are encoded within a genomic island. However, along with proteins that are bona fide and confirmed magnetosome proteins, proteomic studies repetitively revealed the presence of numerous other and partially highly abundant proteins within the magnetosome membrane fraction, which could not yet clearly be discriminated as contaminants, acquired during purification from other cellular compartments, or as genuine integral magnetosome membrane proteins. In order to clearly identify all proteins from the magnetotactic Alphaproteobacterium Magnetospirillum gryphiswaldense that are specifically targeted to the magnetosome membrane and to estimate their abundancy, we analyzed the protein composition of several cellular fractions by semi-quantitative mass spectrometry. Although spectra from over 1000 proteins were found within the magnetosome membrane fraction, our comparative approach could exclude most of them as contaminants. We found that almost all specifically magnetosome membrane-integral proteins belong to a well-defined subgroup of previously identified magnetosome-associated proteins, indicating that the protein composition of the compartment is strongly controlled. Furthermore, the correlation of our semi-quantitative proteome data with recently published quantitative Western blot and cryo-electron tomography results revealed that the magnetosome membrane is tightly packed with transmembrane domains of integral proteins, indicating a very high protein composition of the membrane. Our findings will help to further define the structure of the organelle and contribute to the elucidation of the processes involved in biogenesis of magnetosomes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:04:37.215.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Otto
SpeciesList scientific name: Magnetospirillum gryphiswaldense; NCBI TaxID: 55518;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-24 05:07:55ID requested
12017-10-24 07:14:36announced
22024-10-22 04:04:45announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.jprot.2017.10.007;
Raschdorf O, Bonn F, Zeytuni N, Zarivach R, Becher D, Sch, ΓΌ, ler D, A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle. J Proteomics, 172():89-99(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: organelle, membrane proteome,magnetosome
Contact List
Dr. Andreas Otto
contact affiliationDr. Andreas Otto Institute for Microbiology Department of Microbial Proteomics Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 15 17489 Greifswald
contact emailandreas.otto@uni-greifswald.de
lab head
Andreas Otto
contact affiliationInstitute for Microbiology
contact emailandreas.otto@uni-greifswald.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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