PXD006166

PXD006166 is an original dataset announced via ProteomeXchange.

Title	Towards a quantitative assessment of the integral magnetosome membrane sub-proteome
Description	Magnetosomes are membranous organelles than contain crystals of magnetic minerals and enable magnetotactic bacteria to orientate in magnetic fields. The enveloping magnetosome membrane was found to contain a subset of specific proteins that are encoded within a genomic island. However, along with proteins that are bona fide and confirmed magnetosome proteins, proteomic studies repetitively revealed the presence of numerous other and partially highly abundant proteins within the magnetosome membrane fraction, which could not yet clearly be discriminated as contaminants, acquired during purification from other cellular compartments, or as genuine integral magnetosome membrane proteins. In order to clearly identify all proteins from the magnetotactic Alphaproteobacterium Magnetospirillum gryphiswaldense that are specifically targeted to the magnetosome membrane and to estimate their abundancy, we analyzed the protein composition of several cellular fractions by semi-quantitative mass spectrometry. Although spectra from over 1000 proteins were found within the magnetosome membrane fraction, our comparative approach could exclude most of them as contaminants. We found that almost all specifically magnetosome membrane-integral proteins belong to a well-defined subgroup of previously identified magnetosome-associated proteins, indicating that the protein composition of the compartment is strongly controlled. Furthermore, the correlation of our semi-quantitative proteome data with recently published quantitative Western blot and cryo-electron tomography results revealed that the magnetosome membrane is tightly packed with transmembrane domains of integral proteins, indicating a very high protein composition of the membrane. Our findings will help to further define the structure of the organelle and contribute to the elucidation of the processes involved in biogenesis of magnetosomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:37.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Otto
SpeciesList	scientific name: Magnetospirillum gryphiswaldense; NCBI TaxID: 55518;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-24 05:07:55	ID requested
1	2017-10-24 07:14:36	announced
⏵ 2	2024-10-22 04:04:45	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2017.10.007;

Raschdorf O, Bonn F, Zeytuni N, Zarivach R, Becher D, Sch, ü, ler D, A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle. J Proteomics, 172():89-99(2018) [pubmed]

curator keyword: Biological

submitter keyword: organelle, membrane proteome,magnetosome

Dr. Andreas Otto
contact affiliation	Dr. Andreas Otto Institute for Microbiology Department of Microbial Proteomics Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 15 17489 Greifswald
contact email	andreas.otto@uni-greifswald.de
lab head
Andreas Otto
contact affiliation	Institute for Microbiology
contact email	andreas.otto@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006166

PRIDE project URI

• PRIDE
  1. PXD006166
     1. Label: PRIDE project
     2. Name: Towards a quantitative assessment of the integral magnetosome membrane sub-proteome