PXD006166 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Towards a quantitative assessment of the integral magnetosome membrane sub-proteome |
Description | Magnetosomes are membranous organelles than contain crystals of magnetic minerals and enable magnetotactic bacteria to orientate in magnetic fields. The enveloping magnetosome membrane was found to contain a subset of specific proteins that are encoded within a genomic island. However, along with proteins that are bona fide and confirmed magnetosome proteins, proteomic studies repetitively revealed the presence of numerous other and partially highly abundant proteins within the magnetosome membrane fraction, which could not yet clearly be discriminated as contaminants, acquired during purification from other cellular compartments, or as genuine integral magnetosome membrane proteins. In order to clearly identify all proteins from the magnetotactic Alphaproteobacterium Magnetospirillum gryphiswaldense that are specifically targeted to the magnetosome membrane and to estimate their abundancy, we analyzed the protein composition of several cellular fractions by semi-quantitative mass spectrometry. Although spectra from over 1000 proteins were found within the magnetosome membrane fraction, our comparative approach could exclude most of them as contaminants. We found that almost all specifically magnetosome membrane-integral proteins belong to a well-defined subgroup of previously identified magnetosome-associated proteins, indicating that the protein composition of the compartment is strongly controlled. Furthermore, the correlation of our semi-quantitative proteome data with recently published quantitative Western blot and cryo-electron tomography results revealed that the magnetosome membrane is tightly packed with transmembrane domains of integral proteins, indicating a very high protein composition of the membrane. Our findings will help to further define the structure of the organelle and contribute to the elucidation of the processes involved in biogenesis of magnetosomes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:04:37.215.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Otto |
SpeciesList | scientific name: Magnetospirillum gryphiswaldense; NCBI TaxID: 55518; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-24 05:07:55 | ID requested | |
1 | 2017-10-24 07:14:36 | announced | |
⏵ 2 | 2024-10-22 04:04:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.jprot.2017.10.007; |
Raschdorf O, Bonn F, Zeytuni N, Zarivach R, Becher D, Sch, ΓΌ, ler D, A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle. J Proteomics, 172():89-99(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: organelle, membrane proteome,magnetosome |
Contact List
Dr. Andreas Otto |
contact affiliation | Dr. Andreas Otto Institute for Microbiology Department of Microbial Proteomics Ernst-Moritz-Arndt-University Greifswald F.-L.-Jahn-Str. 15 17489 Greifswald |
contact email | andreas.otto@uni-greifswald.de |
lab head | |
Andreas Otto |
contact affiliation | Institute for Microbiology |
contact email | andreas.otto@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006166
- Label: PRIDE project
- Name: Towards a quantitative assessment of the integral magnetosome membrane sub-proteome