PXD006156

PXD006156 is an original dataset announced via ProteomeXchange.

Title	RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling
Description	RNF8 is a key E3 ubiquitin ligase functioning in both DSB- and UV-induced DDR, identification of additional substrates will help further elucidate its role in DNA damage signaling. To identify substrates of ubiquitin ligases, we have recently devised a method based on proximity-dependent biotin labeling. In this method, an E3 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase BirA together with a biotin acceptor peptide (AP)-tagged ubiquitin. The BirA-directed biotin labeling of AP depends on the proximity of the two fusion proteins in the cell, which leads to preferential labeling of ubiquitinated E3 substrates. In this study, we applied this procedure to RNF8 and identified its substrates.
HostingRepository	PRIDE
AnnounceDate	2018-11-07
AnnouncementXML	Submission_2019-02-12_08:18:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006156
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rakesh Deshar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; GlyGly
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-22 06:21:21	ID requested
1	2018-11-07 01:21:51	announced
⏵ 2	2019-02-12 08:18:42	announced	Updated publication reference for PubMed record(s): 30445466.

Deshar R, Yoo W, Cho EB, Kim S, Yoon JB, RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling. Nucleic Acids Res, 47(2):762-778(2019) [pubmed]

curator keyword: Biological

submitter keyword: RNF8, E3 ligase, substrate identification, DNA damage

Jong-Bok Yoon
contact affiliation	Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Korea. Tel.: +82-2-2123-2704; Fax: 82-2-362- 9897
contact email	yoonj@yonsei.ac.kr
lab head
Rakesh Deshar
contact affiliation	The Catholic University of Korea
contact email	rakeshdesar@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD006156

PRIDE project URI

• PRIDE
  1. PXD006156
     1. Label: PRIDE project
     2. Name: RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling