PXD006155

PXD006155 is an original dataset announced via ProteomeXchange.

Title	An Exported Protein-Interacting Complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes
Description	The human malaria parasite, P. falciparum, generates virulence complexes on the surface of infected red blood cells (RBCs), which include the major virulence antigen Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). The trafficking of PfEMP1 from the parasite to the host cell and its correct display on the RBC surface involves a complement of host and parasite-derived trafficking machineries. In this work we examine the physical organisation of PfEMP1 trafficking intermediates in infected RBCs and determine interacting partners using an epitope-tagged minimal construct (PfEMP1B). We show that PV-located PfEMP1B interacts with components of the Plasmodium Translocon of EXported proteins (PTEX) as well as a novel protein complex we refer to as the Exported Protein-Interacting Complex (EPIC). Within the RBC cytoplasm PfEMP1B interacts with components of the Maurer’s clefts and the RBC chaperonin complex. We define the EPIC interactome and, using an inducible knockdown approach, show that depletion of one of its components, the parasitophorous vacuolar protein-1 (PV1), results in altered knob morphology, reduced cell rigidity and decreased binding of infected RBCs to CD36. Accordingly, we show that deletion of the P. berghei homologue of PV1 is associated with attenuation of parasite virulence in vivo.
HostingRepository	PRIDE
AnnounceDate	2017-07-13
AnnouncementXML	Submission_2017-07-13_03:53:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Batinovic
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-22 04:53:48	ID requested
⏵ 1	2017-07-13 03:53:33	announced

Batinovic S, McHugh E, Chisholm SA, Matthews K, Liu B, Dumont L, Charnaud SC, Schneider MP, Gilson PR, de Koning-Ward TF, Dixon MWA, Tilley L, An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes. Nat Commun, 8():16044(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Plasmodium falciparum, PfEMP1, protein export, PTEX, EPIC

Leann Tilley
contact affiliation	Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne, Parkville, Victoria, Australia
contact email	ltilley@unimelb.edu.au
lab head
Steven Batinovic
contact affiliation	The University of Melbourne
contact email	s.batinovic@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006155
     1. Label: PRIDE project
     2. Name: An Exported Protein-Interacting Complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes