PXD006153

PXD006153 is an original dataset announced via ProteomeXchange.

Title	Metabolite Perception Regulates Colonization and Virulence during Polymicrobial Infection
Description	Many human infections are polymicrobial in origin, and interactions among community inhabitants shape colonization patterns and pathogenic potential1. However, few interspecies interactions have been functionally dissected at the molecular level or characterized on a systems level. Periodontitis, which is the sixth most prevalent infectious disease worldwide2, ensues from the action of dysbiotic polymicrobial communities3. The keystone pathogen Porphyromonas gingivalis and the accessory pathogen Streptococcus gordonii interact to form communities in vitro and exhibit increased fitness in vivo3, 4. The mechanistic basis of this polymicrobial synergy, however, has not been fully elucidated. Here we show that streptococcal 4 aminobenzoate/para-amino benzoic acid (pABA) is required for maximal accumulation of P. gingivalis in dual species communities. Metabolomic and proteomic data showed that exogenous pABA is utilized for folate biosynthesis, and leads to decreased stress and elevated expression of fimbrial interspecies adhesins. Moreover, pABA increased the colonization and survival of P. gingivalis in a murine oral infection model. However, pABA also caused a reduction in virulence in vivo and suppressed extracellular polysaccharide production by P. gingivalis. Collectively, these data reveal a multidimensional aspect to P. gingivalis-S. gordonii interactions and establish pABA as a critical cue produced by a partner species that enhances fitness of P. gingivalis while diminishing virulence.
HostingRepository	PRIDE
AnnounceDate	2017-09-26
AnnouncementXML	Submission_2017-09-26_07:38:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Houser
SpeciesList	scientific name: Porphyromonas gingivalis ATCC 33277; NCBI TaxID: 431947;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-22 03:03:33	ID requested
⏵ 1	2017-09-26 07:38:18	announced

Kuboniwa M, Houser JR, Hendrickson EL, Wang Q, Alghamdi SA, Sakanaka A, Miller DP, Hutcherson JA, Wang T, Beck DAC, Whiteley M, Amano A, Wang H, Marcotte EM, Hackett M, Lamont RJ, Metabolic crosstalk regulates Porphyromonas gingivalis colonization and virulence during oral polymicrobial infection. Nat Microbiol, 2(11):1493-1499(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Porphyromonas gingivalis, P. gingivalis, polymicrobial,Periodontitis, streptococcal 4 aminobenzoate/para-amino benzoic acid (pABA),

Edward Marcotte
contact affiliation	Institute for Cellular and Molecular Biology, and Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, Texas, United States of America
contact email	marcotte@austin.utexas.edu
lab head
John Houser
contact affiliation	University of Texas
contact email	johnrhouser@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006153

PRIDE project URI

• PRIDE
  1. PXD006153
     1. Label: PRIDE project
     2. Name: Metabolite Perception Regulates Colonization and Virulence during Polymicrobial Infection