PXD006152

PXD006152 is an original dataset announced via ProteomeXchange.

Title	TMT analysis of early proteome events in direct cardiomyocyte reprogramming
Description	Direct reprogramming of fibroblasts into cardiomyocyte-like cells (iCM) holds great potential for heart regeneration and disease modeling and may lead to future therapeutic applications in human patients with heart disease. Currently, the application of this technology is limited by our lack of understanding of the molecular mechanisms which drive direct iCM reprogramming. Using a quantitative mass spectrometry-based proteomic approach we have identified the temporal global changes in protein abundance that occur during the initial phases of iCM reprogramming. Collectively, our results show systematic and temporally distinct alterations in the levels of specific functional classes of proteins during the initiating steps of reprogramming including extracellular matrix proteins, translation factors, and chromatin-binding proteins.
HostingRepository	PRIDE
AnnounceDate	2018-02-19
AnnouncementXML	Submission_2018-02-19_02:44:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	T Greco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-22 03:03:08	ID requested
⏵ 1	2018-02-19 02:44:32	announced

Sauls K, Greco TM, Wang L, Zou M, Villasmil M, Qian L, Cristea IM, Conlon FL, Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep, 22(7):1913-1922(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Fibroblast, MEF, GMT, Orbitrap Velos, HCD

Ileana Cristea
contact affiliation	Department of Molecular Biology, Princeton University, USA
contact email	icristea@princeton.edu
lab head
T Greco
contact affiliation	Molecular Biology
contact email	tgreco@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD006152

PRIDE project URI

• PRIDE
  1. PXD006152
     1. Label: PRIDE project
     2. Name: TMT analysis of early proteome events in direct cardiomyocyte reprogramming