PXD006147

PXD006147 is an original dataset announced via ProteomeXchange.

Title	Definition of a high-confidence mitochondrial proteome at quantitative scale_q-AP-MS of mitochondrial protein complexes
Description	Mitochondria perform central functions in cellular bioenergetics, metabolism and signaling and their malfunction has been linked to numerous diseases. The available studies cover only part of the mitochondrial proteome and a separation of core mitochondrial proteins from associated fractions has not been achieved. We developed an integrative, quantitative MS-based experimental approach to define the high confidence proteome of yeast mitochondria and to identify new mitochondrial proteins. The analysis includes protein abundance under fermentable and non-fermentable growth, submitochondrial localization, single-protein analysis and subcellular classification of mitochondria-associated fractions. We identified novel mitochondrial interactors of respiratory chain supercomplexes, ATP synthase, AAA proteases, the mitochondrial contact site and cristae organizing system (MICOS) and coenzyme Q biosynthesis cluster as well as new mitochondrial proteins with dual cellular localization. The integrative proteome provides a high confidence source for the characterization of physiological and pathophysiological functions of mitochondria and their integration into the cellular environment.
HostingRepository	PRIDE
AnnounceDate	2017-07-19
AnnouncementXML	Submission_2017-07-19_06:53:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian D. Peikert
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-21 08:05:29	ID requested
1	2017-06-29 02:02:27	announced
⏵ 2	2017-07-19 06:53:08	announced	Updated project metadata.

Morgenstern M, Stiller SB, L, ü, bbert P, Peikert CD, Dannenmaier S, Drepper F, Weill U, H, ö, ß P, Feuerstein R, Gebert M, Bohnert M, van der Laan M, Schuldiner M, Sch, ü, tze C, Oeljeklaus S, Pfanner N, Wiedemann N, Warscheid B, Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale. Cell Rep, 19(13):2836-2852(2017) [pubmed]

curator keyword: Biological

submitter keyword: SILAC, Saccharomyces cerevisiae, new mitochondrial proteins

Prof. Dr. Bettina Warscheid
contact affiliation	Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany
contact email	Bettina.Warscheid@biologie.uni-freiburg.de
lab head
Christian D. Peikert
contact affiliation	Universität Freiburg, Warscheid Lab
contact email	christian.peikert@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006147

PRIDE project URI

• PRIDE
  1. PXD006147
     1. Label: PRIDE project
     2. Name: Definition of a high-confidence mitochondrial proteome at quantitative scale_q-AP-MS of mitochondrial protein complexes