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PXD006141

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-05-16
  • AnnouncementXML: Submission_2019-05-16_00:13:38.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Dario E. Kalume
  • Title: Exploring the potential role of moonlighting function of the surface-associated proteins from Mycobacterium bovis BCG Moreau and Pasteur by comparative proteomic
  • Description: Tuberculosis (TB) is one of major causes of death worldwide. Bacillus Calmette-Guerin (BCG) is the only licensed TB vaccine and its inability to protect against adult pulmonary TB can be due to genetic differences among strains described since the 1940s. In this work, we compared the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau, the Brazilian vaccine strain, and the BCG Pasteur reference strain. The methodology used was 2D-gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF). We identified 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits.
  • SpeciesList: scientific name: Mycobacterium bovis BCG str. Moreau RDJ; NCBI TaxID: 413996;
  • ModificationList: 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
  • Instrument: 4800 Proteomics Analyzer

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-03-21 04:49:21ID requested
12019-05-15 00:58:19announced
22019-05-16 00:13:39announcedUpdated project metadata.

Publication List

  1. Pagani TD, Guimarães ACR, Waghabi MC, Corrêa PR, Kalume DE, Berrêdo-Pinho M, Degrave WM, Mendonça-Lima L, BCG Moreau and Pasteur by Comparative Proteomic. Front Immunol, 10():716(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Mycobacterium bovis BCG Moreau, moonlighting function, surface-associated proteins

Contact List

    Da¡rio Eluan Kalume
    • contact affiliation: Laboratorio Interdisciplinar de Pesquisas Medicas, LIPMED,Instituto Oswaldo Cruz,IOC,Fundacao Oswaldo Cruz, FIOCRUZ,Rio de Janeiro,Brazil
    • contact email: kalume1@yahoo.com
    • lab head:
    Dario E. Kalume
    • contact affiliation: FIOCRUZ
    • contact email: kalume1@yahoo.com
    • dataset submitter:

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