PXD006136

PXD006136 is an original dataset announced via ProteomeXchange.

Title	SWATH-based quantitative proteomics reveals the mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance in silkworm reared on UV-B treated mulberry leaves
Description	Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most acute infectious diseases in silkworm, which has caused great economic loss in sericulture. Previous study showed that the content of components in mulberry leaves, particularly for moracin N, was increased after UV-B irradiation. In this study, the BmNPV resistance of silkworms reared on UV-B treated and moracin N spreaded mulberry leaves was improved. To uncover the mechanism of enhanced BmNPV resistance, silkworm midguts from UV-B treated mulberry leaves (BUM) and moracin N (BNM) groups were analyzed by SWATH-based proteomic technique. Of note, the abundance of ribosomal proteins in BUM and BNM groups was significantly changed to maintain the synthesis of total protein levels and cell survival. While, cytochrome c oxidase subunit II, calcium ATPase and programmed cell death 4 involved in apoptotic process were up-regulated in BNM group. Expressions of lipase-1, serine protease precursor, Rab1 protein, and histone genes were increased significantly in BNM group. These results suggest that moracin N might be the main active components in UV-B treated mulberry leaves to affect the BmNPV-resistance of silkworm, which could promote apoptotic cell death, enhance the organism immunity, and regulate the intercellular environment of cells in silkworm. It also presents an innovative process to reduce the mortality rate of silkworm infected with BmNPV.
HostingRepository	PRIDE
AnnounceDate	2017-06-01
AnnouncementXML	Submission_2017-06-01_06:00:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Zhu
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-21 02:57:59	ID requested
⏵ 1	2017-06-01 06:00:15	announced

Hu J, Zhu W, Li Y, Guan Q, Yan H, Yu J, Fu Z, Lu X, Tian J, SWATH-based quantitative proteomics reveals the mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance in silkworm reared on UV-B treated mulberry leaves. Proteomics, 17(13-14):(2017) [pubmed]

curator keyword: Biological

submitter keyword: silkworm (Bombyx mori)

proteomics

UV-B treated mulberry leaves

moracin N;

Jingkui Tian
contact affiliation	College of Biomedical Engineering & Instrument Science,Zhejiang University
contact email	rutin@zju.edu.cn
lab head
Wei Zhu
contact affiliation	Zhejiang University
contact email	rutin@zju.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006136
     1. Label: PRIDE project
     2. Name: SWATH-based quantitative proteomics reveals the mechanism of enhanced Bombyx mori nucleopolyhedrovirus-resistance in silkworm reared on UV-B treated mulberry leaves