PXD006126

PXD006126 is an original dataset announced via ProteomeXchange.

Title	Inhibition of melanization activation by Serpin-5 and Serpin-9 promotes nucleopolyhedrovirus infection in cotton bollworm Helicoverpa armigera
Description	One of important insect defense mechanisms is melanization, which are mediated by clip domain serine protease (cSP) cascades and regulated by Serpins. In vitro, activation of melanization can efficiently kill Nucleopolyhedrovirus (NPV). However, quantitative proteomics revealed that the infection of NPV in cotton bollworm Helicoverpa armigera suppressed protein levels of melanization components in the host hemolymph. It also reduced the prophenoloxidase (PPO) activity. In contrast, Serpin-9 and -5 were sequentially up-regulated. Serpin-5 and -9 induced by NPV infection play important roles in regulate host melanization by directly inhibiting their target proteases cSP4 and cSP6 respectively. Furthermore, Serpin-5 or -9 depleted insects exhibited high PO activities and show resistance to NPV infection. Together, our results characterized melanization cascade in H. armigera, and suggests that natural insect virus NPV has evolved a distinct strategy to suppress host immune system. This finding may be exploited to design more potent viruses against agricultural pests.
HostingRepository	PRIDE
AnnounceDate	2017-09-19
AnnouncementXML	Submission_2018-05-23_05:48:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Zou
SpeciesList	scientific name: Helicoverpa armigera (Cotton bollworm); NCBI TaxID: 29058;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-20 06:39:47	ID requested
1	2017-09-19 00:21:55	announced
⏵ 2	2018-05-23 05:48:06	announced	Updated publication reference for PubMed record(s): 28953952.

Yuan C, Xing L, Wang M, Wang X, Yin M, Wang Q, Hu Z, Zou Z, Inhibition of melanization by serpin-5 and serpin-9 promotes baculovirus infection in cotton bollworm Helicoverpa armigera. PLoS Pathog, 13(9):e1006645(2017) [pubmed]

curator keyword: Biological

submitter keyword: Melanization, Prophenoloxidase, Serpin, Cotton bollworm, Hemolymph

Zhen Zou
contact affiliation	Institute of Zoology, Chinese Academy of Sciences
contact email	zouzhen@ioz.ac.cn
lab head
Zhen Zou
contact affiliation	Institute of Zoology, CAS
contact email	zouzhen@ioz.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006126
     1. Label: PRIDE project
     2. Name: Inhibition of melanization activation by Serpin-5 and Serpin-9 promotes nucleopolyhedrovirus infection in cotton bollworm Helicoverpa armigera