PXD006121

PXD006121 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesis
Description	DNA processing chain A (DprA) is a DNA binding protein which is ubiquitous in bacteria and required for DNA transformation. However, the interaction of DprA with competence proteins and recombination is poorly understood. Therefore, the proteomes of whole Neisseria meningitidis (Nm) wildtype and dprA mutant cells were compared. Such a comparative proteomic analysis increases our understanding of the interactions of DprA with other Nm components, and may elucidate its potential role beyond DNA processing in transformation. Using label-free quantitative proteomics, a total of 1057 unique Nm proteins were identified out of which 100 were quantified as differentially abundant (P ≤ 0.05 and fold change ≥ |2|) in the dprA null mutant. Proteins involved in homologous recombination (RecA, UvrD and HolA), pilus biogenesis (PilG, PilT1, PilT2, PilM, PilO, PilQ, PilF and PilE), and cell division, including core energy metabolism and response to oxidative stress were downregulated in dprA null mutant Nm. Immunoblotting was employed to validate the differences observed in two selected proteins. The analysis revealed reduced amounts of PilG in the dprA null mutant and reduced amounts of DprA in the Nm pilG null mutant. In conclusion, DprA interacts with proteins essential for Nm DNA recombination in transformation, pilus biogenesis and other functions associated with the inner membrane. Inverse downregulation of Nm DprA and PilG expression in the corresponding mutants indicate a link between DNA processing and pilus biogenesis demonstrated for the first time.
HostingRepository	PRIDE
AnnounceDate	2017-04-26
AnnouncementXML	Submission_2017-04-26_05:10:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shewit Kalayou
SpeciesList	scientific name: Neisseria meningitidis serogroup B; NCBI TaxID: 491;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-20 04:50:02	ID requested
⏵ 1	2017-04-26 05:10:57	announced

Beyene GT, Kalayou S, Riaz T, Tonjum T, Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesis. BMC Microbiol, 17(1):96(2017) [pubmed]

curator keyword: Biological

submitter keyword: Neisseria meningitides, DNA processing, pilus biogenesis, DprA, PilG, proteomics, mass spectrometry

Prof Tone Tønjum
contact affiliation	Department of Microbiology, Institute of Clinical medicine, University of Oslo, Norway.
contact email	tone.tonjum@medisin.uio.no
lab head
Shewit Kalayou
contact affiliation	Genome dynamics group, Oslo University Hospital
contact email	shetek@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006121
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesis